Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294

Product Name

Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294

View all EGFR Antibodies

SKU/Catalog Number

M00023-3

Size

100uL

Form

Liquid

Description

Boster Bio Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294 (Catalog # M00023-3). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EGF Receptor Rabbit Monoclonal Antibody, Clone#RM294 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00023-3)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM294

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the C-terminus of human EGF Receptor (c-ErbB-1).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human EGF Receptor (c-ErbB-1). No cross-reactivity with HER2, HER3, or HER4

Reactive Species

M00023-3 is reactive to EGFR in Human

Applications

M00023-3 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

134277 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:200-1:500 dilution.

Validation Images & Assay Conditions

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Figure 1. Western Blotting result
Western Blot of HeLa cell lysates using anti-EGF Receptor rabbit monoclonal antibody (clone RM294) at a 1:200 dilution.

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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-EGF Receptor rabbit monoclonal antibody (clone RM294) at a 1:200 dilution.

Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1

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