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Product Info Summary
| SKU: | A03115 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ECP Antibody
SKU/Catalog Number
A03115
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ECP Antibody catalog # A03115. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ECP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03115)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ECP. AA range:102-151
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03115 is reactive to RNASE3 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
18385 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03115 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of various cells using ECP Polyclonal Antibody
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Western blot analysis of lysate from 293 cells, usiung ECP antibody.
Protein Target Info & Infographic
Gene/Protein Information For RNASE3 (Source: Uniprot.org, NCBI)
Gene Name
RNASE3
Full Name
Eosinophil cationic protein
Weight
18385 MW
Superfamily
pancreatic ribonuclease family
Alternative Names
Eosinophil cationic protein;ECP;3.1.27.-;Ribonuclease 3;RNase 3;RNASE3;ECP, RNS3; RNASE3 ECP, RAF1, RNS3 ribonuclease A family member 3 eosinophil cationic protein|RNase 3|cytotoxic ribonuclease|ribonuclease 3|ribonuclease, RNase A family, 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNASE3, check out the RNASE3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNASE3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ECP Antibody (A03115)
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No publications found for A03115
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-ECP Antibody
Question
Would anti-ECP antibody A03115 work on dog WB with bone marrow?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
Our lab technicians have not validated anti-ECP antibody A03115 on dog. You can run a BLAST between dog and the immunogen sequence of anti-ECP antibody A03115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog bone marrow in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-07
Question
I have a question about product A03115, anti-ECP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03115 anti-ECP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-28
Question
Does A03115 anti-ECP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, A03115 anti-ECP antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-11
Question
Is there a BSA free version of anti-ECP antibody A03115 available?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ECP antibody A03115 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-ECP antibody (A03115)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We do provide the blocking peptide for product anti-ECP antibody (A03115). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-29
Question
Does anti-ECP antibody A03115 work for WB with colon?
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
According to the expression profile of colon, RNASE3 is highly expressed in colon. So, it is likely that anti-ECP antibody A03115 will work for WB with colon.
Boster Scientific Support
Answered: 2019-04-01
Question
I see that the anti-ECP antibody A03115 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-15
