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Product Info Summary
| SKU: | A03795-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PFAS Antibody Picoband®
SKU/Catalog Number
A03795-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PFAS Antibody Picoband® catalog # A03795-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PFAS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03795-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PFAS recombinant protein (Position: R330-S569).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03795-1 is reactive to PFAS in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
145 kDa
Calculated molecular weight
64133 MW
Background of PURL
Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03795-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human placenta tissue, human A549 whole cell, human Hek293 whole cell, human Hela whole cell, human K562 whole cell, human U937 whole cell, human HepG2 whole cell, rat liver tissue, rat brain tissue, rat testis tissue, mouse liver tissue, mouse brain tissue, mouse testis tissue
ICC/IF: HELA cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PFAS using anti-PFAS antibody (A03795-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human Hek293 whole cell lysates,
Lane 4: human Hela whole cell lysates,
Lane 5: human K562 whole cell lysates,
Lane 6: human U937 whole cell lysates,
Lane 7: human HepG2 whole cell lysates,
Lane 8: rat liver tissue lysates,
Lane 9: rat brain tissue lysates,
Lane 10: rat testis tissue lysates,
Lane 11: mouse liver tissue lysates,
Lane 12: mouse brain tissue lysates,
Lane 12: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFAS antigen affinity purified polyclonal antibody (Catalog # A03795-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFAS at approximately 145KD. The expected band size for PFAS is at 145KD.
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Figure 2. IF analysis of PFAS using anti-PFAS antibody (A03795-1).
PFAS was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-PFAS Antibody (A03795-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HepG2 cells using anti-PFAS antibody (A03795-1).
Overlay histogram showing HepG2 cells stained with A03795-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PFAS Antibody (A03795-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For PFAS (Source: Uniprot.org, NCBI)
Gene Name
PFAS
Full Name
Phosphoribosylformylglycinamidine synthase
Weight
64133 MW
Alternative Names
S-arrestin; 48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin; SAG; PFAS FGAMS, FGAR-AT, FGARAT, GATD8, PURL phosphoribosylformylglycinamidine synthase phosphoribosylformylglycinamidine synthase|FGAM synthase|FGAR amidotransferase|IKZF1/PFAS fusion|formylglycinamide ribonucleotide amidotransferase|formylglycinamide ribotide amidotransferase|formylglycinamide ribotide synthetase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PFAS, check out the PFAS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PFAS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PFAS Antibody Picoband® (A03795-1)
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