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Product Info Summary
| SKU: | M01234 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Dysferlin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01234
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Dysferlin Rabbit Monoclonal Antibody catalog # M01234. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dysferlin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01234)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EGO-4
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Dysferlin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01234 is reactive to DYSF in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
280 kDa
Calculated molecular weight
237295 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01234 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: mouse skeletal muscle tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Dysferlin using anti-Dysferlin antibody (M01234).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dysferlin antigen affinity purified monoclonal antibody (Catalog # M01234) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dysferlin at approximately 280 kDa. The expected band size for Dysferlin is at 237 kDa.
Protein Target Info & Infographic
Gene/Protein Information For DYSF (Source: Uniprot.org, NCBI)
Gene Name
DYSF
Full Name
Dysferlin
Weight
237295 MW
Superfamily
ferlin family
Alternative Names
Dysferlin;Dystrophy-associated fer-1-like protein;Fer-1-like protein 1;DYSF;FER1L1; DYSF FER1L1, LGMD2B, LGMDR2, MMD1 dysferlin dysferlin|dystrophy-associated fer-1-like 1|fer-1-like family member 1|fer-1-like protein 1|limb girdle muscular dystrophy 2B (autosomal recessive)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DYSF, check out the DYSF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DYSF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dysferlin Rabbit Monoclonal Antibody (M01234)
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1 Customer Q&As for Anti-Dysferlin Rabbit Monoclonal Antibody
Question
We are currently using anti-Dysferlin Rabbit Monoclonal antibody M01234 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
The anti-Dysferlin Rabbit Monoclonal antibody (M01234) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-04
