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Product Info Summary
| SKU: | A00325 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Mouse, Rat, Primate |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HDAC-2 (C-terminus) Antibody
View all Histone Deacetylase 2/HDAC2 Antibodies
SKU/Catalog Number
A00325
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-HDAC-2 (C-terminus) Antibody (Catalog # A00325). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Primate.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening. (Ship on dry ice.)
Cite This Product
Anti-HDAC-2 (C-terminus) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00325)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Clonality
Polyclonal
Isotype
IgG
Immunogen
HDAC2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus region of human HDAC2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00325 is reactive to HDAC2 in Human, Mouse, Rat, Primate
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
55364 MW
Background of Histone Deacetylase 2/HDAC2
Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. Anti-HDAC2 antibodies are ideal for researchers interested in Breast Cancer, Cancer, Chromatin Research, Epigenetics, Histone Deacetylases, and Stem Cell Markers research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00325 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:20,000 - 1:60,000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 1 µg/ml
Validation Images & Assay Conditions
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Western blot analysis of HDAC2 expression in mouse brain extract (lane 1) and mouse brain extract blocked with peptide (lane 2). HDAC2 at 59KD was detected using rabbit anti-HDAC-2 Antigen Affinity purified polyclonal antibody (Catalog # A00325) at 1 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For HDAC2 (Source: Uniprot.org, NCBI)
Gene Name
HDAC2
Full Name
Histone deacetylase 2
Weight
55364 MW
Superfamily
histone deacetylase family
Alternative Names
HDAC-2, HDAC2, HDAC 2, HD 2, histone deacetylase 2, Histone deacetylase-2, HD2 HDAC2 HD2, KDAC2, RPD3, YAF1 histone deacetylase 2 histone deacetylase 2|YY1-associated factor 1|transcriptional regulator homolog RPD3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HDAC2, check out the HDAC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HDAC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HDAC-2 (C-terminus) Antibody (A00325)
Hello CJ!
A00325 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen Xm, Xie Xb, Zhao Q, Wang F, Bai Y, Yin Jq, Jiang H, Xie Xl, Jia Q, Huang G. Mol Med Rep. 2015 Jan;11(1):105-12. Doi: 10.3892/Mmr.2014.2733. Epub 2014 Oct 21. Ampelopsin Induces Apoptosis By Regulating Multiple C-Myc/S-Phase Kinase-Associated ...
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