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Product Info Summary
| SKU: | A00410-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DR5/Tnfrsf10b Antibody Picoband®
View all TRAILR2/TNFRSF10B Antibodies
SKU/Catalog Number
A00410-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-DR5/Tnfrsf10b Antibody Picoband® catalog # A00410-4. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DR5/Tnfrsf10b Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00410-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse DR5/Tnfrsf10b recombinant protein (Position: Q63-A174).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A00410-4 is reactive to Tnfrsf10b in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
48 kDa
Calculated molecular weight
47878 MW
Background of TRAILR2/TNFRSF10B
Predicted to enable TRAIL receptor activity; identical protein binding activity; and protease binding activity. Predicted to be involved in TRAIL-activated apoptotic signaling pathway and positive regulation of apoptotic process. Predicted to act upstream of or within apoptotic process and regulation of apoptotic process. Predicted to be located in Golgi apparatus; cytosol; and membrane raft. Predicted to be active in cell surface and plasma membrane. Is expressed in bladder; liver; renal vasculature; urethra of female; and urethra of male. Human ortholog(s) of this gene implicated in carcinoma (multiple); cervical cancer; hematologic cancer (multiple); and urinary bladder cancer. Orthologous to several human genes including TNFRSF10A (TNF receptor superfamily member 10a).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00410-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
ELISA, 0.1-0.5 µg/ml, Mouse
Positive Control
WB: rat brain tissue, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DR5/Tnfrsf10b using anti-DR5/Tnfrsf10b antibody (A00410-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates,
Lane 3: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DR5/Tnfrsf10b antigen affinity purified polyclonal antibody (Catalog # A00410-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DR5/Tnfrsf10b at approximately 48 kDa. The expected band size for DR5/Tnfrsf10b is at 48 kDa.
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf10b (Source: Uniprot.org, NCBI)
Gene Name
Tnfrsf10b
Full Name
Tumor necrosis factor receptor superfamily member 10B
Weight
47878 MW
Alternative Names
Tumor necrosis factor receptor superfamily member 10B;Death receptor 5;TNF-related apoptosis-inducing ligand receptor 2;TRAIL receptor 2;TRAIL-R2;CD262;TNFRSF10B;DR5, KILLER, TRAILR2, TRICK2, ZTNFR9;UNQ160/PRO186; TNFRSF10B CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNF receptor superfamily member 10b tumor necrosis factor receptor superfamily member 10B|Fas-like protein|TNF-related apoptosis-inducing ligand receptor 2|apoptosis inducing protein TRICK2A/2B|apoptosis inducing receptor TRAIL-R2|cytotoxic TRAIL receptor-2|death domain containing receptor for TRAIL/Apo-2L|death receptor 5|p53-regulated DNA damage-inducible cell death receptor(killer)|tumor necrosis factor receptor superfamily, member 10b|tumor necrosis factor receptor-like protein ZTNFR9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tnfrsf10b, check out the Tnfrsf10b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tnfrsf10b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DR5/Tnfrsf10b Antibody Picoband® (A00410-4)
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