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Product Info Summary
| SKU: | A04214-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-DNMT3L Antibody Picoband®
SKU/Catalog Number
A04214-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DNMT3L Antibody Picoband® catalog # A04214-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-DNMT3L Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04214-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DNMT3L recombinant protein (Position: M1-L386).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04214-3 is reactive to DNMT3L in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
87794 MW
Background of DNMT3L
DNA (cytosine-5)-methyltransferase 3-like is an enzyme that in humans is encoded by the DNMT3L gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04214-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat testis tissue, rat kidney tissue, mouse testis tissue, mouse kidney tissue
FCM: Caco-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DNMT3L using anti-DNMT3L antibody (A04214-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse testis tissue lysates,
Lane 4: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNMT3L antigen affinity purified polyclonal antibody (Catalog # A04214-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNMT3L at approximately 44 kDa. The expected band size for DNMT3L is at 44 kDa.
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Figure 2. Flow Cytometry analysis of Caco-2 cells using anti-DNMT3L antibody (A04214-3).
Overlay histogram showing Caco-2 cells stained with A04214-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNMT3L Antibody (A04214-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For DNMT3L (Source: Uniprot.org, NCBI)
Gene Name
DNMT3L
Full Name
DNA (cytosine-5)-methyltransferase 3-like
Weight
87794 MW
Alternative Names
Transforming acidic coiled-coil-containing protein 1;Gastric cancer antigen Ga55;Taxin-1;TACC1;KIAA1103; DNMT3L DNA methyltransferase 3 like DNA (cytosine-5)-methyltransferase 3-like|DNA (cytosine-5-)-methyltransferase 3-like|cytosine-5-methyltransferase 3-like protein|human cytosine-5-methyltransferase 3-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNMT3L, check out the DNMT3L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNMT3L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNMT3L Antibody Picoband® (A04214-3)
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