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- Table of Contents
Facts about DNA (cytosine-5)-methyltransferase 3-like.
Acts by hastening the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex following DNA binding to the methyltransferases (PubMed:17687327). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by activation or recruitment of DNMT3 (PubMed:17687327).
Human | |
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Gene Name: | DNMT3L |
Uniprot: | Q9UJW3 |
Entrez: | 29947 |
Belongs to: |
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No superfamily |
DNA (cytosine-5-)-methyltransferase 3-like; DNA (cytosine-5)-methyltransferase 3-like; human cytosine-5-methyltransferase 3-like protein, 10cytosine-5-methyltransferase 3-like protein; MGC1090
Mass (kDA):
43.583 kDA
Human | |
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Location: | 21q22.3 |
Sequence: | 21; NC_000021.9 (44246339..44261897, complement) |
Expressed at low levels in several tissues including testis, ovary, and thymus.
Nucleus.
This article gives a summary of Boster Bio's gene infographics. I also explain how AlbIX helped improve D4 blastocyst development. Finally, I highlight some of the most effective uses of the DNMT3L marker. Continue reading to learn more. Also, be sure to check out Boster's other infographics on genes. These can help you choose the most suitable gene for your study.
L'Gln and L'Pro significantly increased blastocysts when in isosmotic condition than high-density cells that did not contain amino acids. However, these amino acids had no effect on the average number cells in blastocysts. This was a new finding for embryonic stem cells. This may also be a novel approach to stimulate D4 blastocyst growth.
After insemination, embryos were transferred into sequential cultures that contained one concentration of Triton X-100, a control. The AlbIX group received less human albumin recombinant, whereas the control group had an elevated concentration. After 48 hours, embryos were transferred into an additional culture medium that contained less AlbIX. The embryos were then examined using fluorescence microscopy using MetaMorph software.
Despite its lack of influence on the quantity of cleaved oocytes, TX100 blocked the development of D4 blastocysts. However the high-concentration AlbIX reduced the proportion of embryos cleaved reaching the blastocyst stage. This suggests that AlbIX could be a viable candidate to treat embryonic stem cells.
LAH significantly improved blastocyst usability compared to D4 LGCEs. The treatment also increased the cancellation rate. These results together suggest a very efficient method to increase the growth of blastocysts. This could lead to better outcomes for embryonic stem cells lines and reduce repeat cycles. It is important to remember that AlbIX is just one of many treatments that can improve D4 blastocyst development.
LAH also significantly increased the proportion of total blastocysts found in LGCEs. D4 blastocysts growth was improved by LAH. This is due to the release of ROS from embryos. This reduces the number of undesirable substances. Furthermore, LAH improved the formation of early hatching from the zona pellucida. The use of LAH in LGCEs could have a positive impact on the rates of clinical pregnancy.
L-Pro, Gly and Betaine did do not improve D4 blastocyst formation in low density cultures However, they did increase hatching rates in HD cultures. L-Pro and L'Gln were not affected by molar excess Betaine. It appears that L-Pro and Gly could enhance D4 blastocyst development but reduce the number of cells.
The DNMT3L DNA methylation marker is a sensitive test that checks the status of DNA methylation inside ESCs. It is made of rabbit polyclonal antibodies. There are many types of antibodies that can be used for this test. There are several types of antibodies available for this test, including the anti-alpha Tubulin (anti-GFP), Anti-mouse anti-GFP, anti xUHRF1, and anti-xUHRF1 rabbit polyclonal anti–UHRF1.
PMID: 10857753 by Aapola U., et al. Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family.
PMID: 17687327 by Ooi S.K., et al. DNMT3L connects unmethylated lysine 4 of histone H3 to de novo methylation of DNA.