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Product Info Summary
| SKU: | A30641 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-DMGDH Antibody
SKU/Catalog Number
A30641
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DMGDH Antibody (Catalog# A30641). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DMGDH Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30641)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human DMGDH. Immunogen sequence location: 817-866
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30641 is reactive to DMGDH in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
96811 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30641 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from HT-29 and A549 cells, using DMGDH Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For DMGDH (Source: Uniprot.org, NCBI)
Gene Name
DMGDH
Full Name
Dimethylglycine dehydrogenase, mitochondrial
Weight
96811 MW
Superfamily
GcvT family
Alternative Names
DMGDH;Dimethylglycine dehydrogenase; mitochondrial;ME2GLYDH DMGDH DMGDHD, ME2GLYDH dimethylglycine dehydrogenase dimethylglycine dehydrogenase, mitochondrial
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DMGDH, check out the DMGDH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DMGDH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DMGDH Antibody (A30641)
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Customer Q&As
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5 Customer Q&As for Anti-DMGDH Antibody
Question
See attached the WB image, lot number and protocol we used for synovial cell trachea using anti-DMGDH antibody A30641. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-05
Question
I was wanting to use your anti-DMGDH antibody for WB for human synovial cell trachea on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human synovial cell trachea identification?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
You can see on the product datasheet, A30641 anti-DMGDH antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human synovial cell trachea in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-20
Question
We are currently using anti-DMGDH antibody A30641 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
The anti-DMGDH antibody (A30641) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-17
Question
My question regarding product A30641, anti-DMGDH antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30641 anti-DMGDH antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-03
Question
My lab would like to test anti-DMGDH antibody A30641 on human synovial cell trachea for research purposes, then I may be interested in using anti-DMGDH antibody A30641 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Moore
Verified customer
Asked: 2018-08-29
Answer
The products we sell, including anti-DMGDH antibody A30641, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-29
