Anti-PKC Epsilon PRKCE Antibody

Product Name

Anti-PKC Epsilon PRKCE Antibody

View all PKC epsilon Antibodies

SKU/Catalog Number

A01151

Size

100ul

Form

Liquid

Description

Boster Bio Anti-PKC Epsilon PRKCE Antibody catalog # A01151. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PKC Epsilon PRKCE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01151)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human C11B2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01151 is reactive to PRKCE in Human, Mouse, Rat

Applications

A01151 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

83674 MW

Background of PKC epsilon

This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na+ ions may pass in accordance with their electrochemical gradient. This sodium channel may be present in both denervated and innervated skeletal muscle.

George A.L. Jr., Ann. Neurol. 31:131-137(1992).
Wang J., Biochem. Biophys. Res. Commun. 182:794-801(1992).
Tsujino A., Proc. Natl. Acad. Sci. U.S.A. 100:7377-7382(2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of PRKCE using Anti-PKC Epsilon PRKCE Antibody (A01151).

ICC staining PKC epsilon in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-PKC Epsilon PRKCE Antibody A01151

Western blot analysis of PKC epsilon on different cell lysate using anti-PKC epsilon antibody at 1/1

Gene/Protein Information For PRKCE (Source: Uniprot.org, NCBI)

Alternative Names

EC 2.7.11; EC 2.7.11.13; MGC125656; nPKC-epsilon; PKC epsilon; PKCE; PKCEMGC125657; PRKCE; protein kinase C epsilon type; protein kinase C, epsilon PRKCE PKCE, nPKC-epsilon protein kinase C epsilon protein kinase C epsilon type

For more info on PRKCE, check out the PRKCE Infographic

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In this infographic, you will see the following information for PRKCE: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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