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Product Info Summary
| SKU: | A12426 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-Diacylglycerol kinase kappa DGKK Antibody
SKU/Catalog Number
A12426
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Diacylglycerol kinase kappa DGKK Antibody (Catalog # A12426). Tested in IHC, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Diacylglycerol kinase kappa DGKK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12426)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human DGKK.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12426 is reactive to DGKK in Human
Reconstitution
Calculated molecular weight
141829 MW
Background of DGKK
Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA).
Imai S., J. Biol. Chem. 280:39870-39881(2005).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12426 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DGKK antibody A12426.
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Immunofluorescence analysis of COS-7 cells, using DGKK antibody A12426.
Protein Target Info & Infographic
Gene/Protein Information For DGKK (Source: Uniprot.org, NCBI)
Gene Name
DGKK
Full Name
Diacylglycerol kinase kappa
Weight
141829 MW
Superfamily
eukaryotic diacylglycerol kinase family
Alternative Names
Diacylglycerol kinase kappa;DAG kinase kappa;2.7.1.107;142 kDa diacylglycerol kinase;Diglyceride kinase kappa;DGK-kappa;DGKK; DGKK diacylglycerol kinase kappa diacylglycerol kinase kappa|142 kDa diacylglycerol kinase|DAG kinase kappa|DGK-kappa|diglyceride kinase kappa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DGKK, check out the DGKK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DGKK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Diacylglycerol kinase kappa DGKK Antibody (A12426)
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No publications found for A12426
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Diacylglycerol kinase kappa DGKK Antibody
Question
I was wanting to use your anti-DGKK antibody for IHC for human metanephros on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human metanephros identification?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
It shows on the product datasheet, A12426 anti-DGKK antibody has been validated for IF, IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human metanephros in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
See attached the WB image, lot number and protocol we used for metanephros using anti-DGKK antibody A12426. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-06
Question
Can you help my question with product A12426, anti-DGKK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12426 anti-DGKK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-22
Question
Is this A12426 anti-DGKK antibody reactive to the isotypes of DGKK?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The immunogen of A12426 anti-DGKK antibody is Synthesized peptide derived from C-terminal of human DGKK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-23
Question
We are currently using anti-DGKK antibody A12426 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The anti-DGKK antibody (A12426) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-02
Question
Is a blocking peptide available for product anti-DGKK antibody (A12426)?
C. Thomas
Verified customer
Asked: 2015-08-31
Answer
We do provide the blocking peptide for product anti-DGKK antibody (A12426). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-08-31
Question
I am looking for to test anti-DGKK antibody A12426 on human metanephros for research purposes, then I may be interested in using anti-DGKK antibody A12426 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Rodriguez
Verified customer
Asked: 2014-01-01
Answer
The products we sell, including anti-DGKK antibody A12426, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-01
