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Product Info Summary
| SKU: | A03671 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-DFF45 DFFA Antibody
View all DFF45/ICAD Antibodies
SKU/Catalog Number
A03671
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-DFF45 DFFA Antibody (Catalog # A03671). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
DFF45 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-DFF45 DFFA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03671)
Host
Rabbit
Contents
DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45. The immunogen is located within the last 50 amino acids of DFF45.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three isoforms of KIF5 are known to exist; this antibody will recognize all three isoforms.
Reactive Species
A03671 is reactive to DFFA in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
36522 MW
Background of DFF45/ICAD
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03671 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DFF45 in HeLa (H)
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Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 μg/mL.
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Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 μg/mL.
Green: DFF45 Antibody (A03671)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For DFFA (Source: Uniprot.org, NCBI)
Gene Name
DFFA
Full Name
DNA fragmentation factor subunit alpha
Weight
36522 MW
Alternative Names
DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit DFFA DFF-45, DFF1, ICAD DNA fragmentation factor subunit alpha DNA fragmentation factor subunit alpha|DFF45|DNA fragmentation factor 45 kDa subunit|DNA fragmentation factor, 45kDa, alpha polypeptide|inhibitor of CAD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DFFA, check out the DFFA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DFFA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DFF45 DFFA Antibody (A03671)
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