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Product Info Summary
| SKU: | EK1564 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1564
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat WIF1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Wif1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1564)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G29-W379
Sensitivity
<10 pg/ml
Assay Range
23.4 pg/ml - 1500 pg/ml
Standard Dilution Instructions
See datasheet of EK1564 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1564 is reactive to Wif1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1564 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of WIF-1
Wnt inhibitory factor 1 is a protein that in humans is encoded by the WIF1 gene. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Additionally, this protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1564-CAP | Anti-Rat Wif1 Pre-coated 96-well strip microplate | 1 |
| EK1564-ST | Rat Wif1 Standard | 2 vials, 10 ng/tube |
| EK1564-DA | Rat Wif1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 23.4 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 |
| O.D. | 0.161 | 0.207 | 0.211 | 0.303 | 0.440 | 0.729 | 1.266 | 2.369 |
Data Example Images
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Rat WIF1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect WIF-1, Dilution ratio of 1:1, concentration in serum and plasma is around 190 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 46 | 179 | 730 | 48 | 172 | 703 |
| Standard deviation | 3.49 | 10.2 | 32.85 | 4.41 | 10.32 | 44.28 |
| CV (%) | 7.6% | 5.7% | 4.5% | 9.2% | 6% | 6.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of WIF-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 46 | 51 | 47 | 44 | 47 | 2.54 | 5.4% |
| Sample 2 | 179 | 201 | 178 | 187 | 186 | 9.2 | 4.9% |
| Sample 3 | 730 | 656 | 670 | 709 | 691 | 29.62 | 4.2% |
Protein Target Info & Infographic
Gene/Protein Information For Wif1 (Source: Uniprot.Org, NCBI)
Gene Name
Wif1
Full Name
Wnt inhibitory factor 1
Weight
41585 MW
Alternative Names
Wnt inhibitory factor 1;WIF-1;Wif1; WIF1 WIF-1 WNT inhibitory factor 1 wnt inhibitory factor 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Wif1, check out the Wif1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Wif1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine® (EK1564)
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Customer Q&As
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8 Customer Q&As for Rat WIF1/Wnt inhibitory factor 1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-06-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-06-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Huang
Verified customer
Asked: 2020-01-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-01-20
Question
Q: can you recommend the dilution ratio of serum samples for detection of WIF-1 in Rat cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
P. Mehta
Verified customer
Asked: 2019-10-01
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the WIF-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-01
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-01
Question
Q: how can I thaw whole blood sample for WIF-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-02-24
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test WIF-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-02-24
Question
Q: What is the optimal O.D. value for WIF-1 ELISA kit? I used your WIF-1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain WIF-1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-11-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this WIF-1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of WIF-1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-11-09
Question
Q: if the enzyme conjugated WIF-1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the WIF-1 antigen?
Verified Customer
Verified customer
Asked: 2016-12-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-12-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification WIF-1 using the EK1564 Rat WIF-1 Picokine® ELISA Kit?
S. Tiwari
Verified customer
Asked: 2016-04-09
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2016-04-09
