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Product Info Summary
| SKU: | M00978 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-CLPX Antibody Picoband® (monoclonal, 11C11)
SKU/Catalog Number
M00978
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CLPX Antibody Picoband® (monoclonal, 11C11) catalog # M00978. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLPX Antibody Picoband® (monoclonal, 11C11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00978)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
11C11
Isotype
Mouse IgG2b
Immunogen
E. coli-derived human CLPX recombinant protein (Position: A337-E574).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00978 is reactive to CLPX in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
69 kDa
Calculated molecular weight
69.224kDa
Background of CLPX
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial is an enzyme that in humans is encoded by the CLPX gene. This protein is a member of the family of AAA Proteins (AAA+ ATPase) and is to form the protein complex of Clp protease. The protein encoded by this gene is part of a protease found in mitochondria. This protease is ATP-dependent and targets specific proteins for degradation. The protease consists of two heptameric rings of the CLPP catalytic subunit sandwiched between two hexameric rings of the chaperone subunit encoded by this gene. Targeted proteins are unwound by this protein and then passed on to the CLPP subunit for degradation. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00978 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Positive Control
WB: human Raji tissue, human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell,
ICC/IF: MCF7 cell
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CLPX using anti ZO-1 antibody (M00978).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji tissue lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CLPX antigen affinity purified polyclonal antibody (Catalog # M00978) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CLPX at approximately 69KD. The expected band size for CLPX is at 69KD.
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Figure 2. IF analysis of CLPX using anti-CLPX antibody (M00978).
CLPX was detected in immunocytochemical section of MCF7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-CLPX Antibody (M00978) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CLPX (Source: Uniprot.org, NCBI)
Gene Name
CLPX
Full Name
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial
Weight
69.224kDa
Superfamily
ClpX chaperone family
Alternative Names
ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; CLPX CLPX EPP2 caseinolytic mitochondrial matrix peptidase chaperone subunit X ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial|ClpX caseinolytic peptidase X homolog|ClpX caseinolytic protease X homolog|energy-dependent regulator of proteolysis
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLPX, check out the CLPX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLPX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLPX Antibody Picoband® (monoclonal, 11C11) (M00978)
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Customer Q&As
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2 Customer Q&As for Anti-CLPX Antibody Picoband® (monoclonal, 11C11)
Question
Has M00978 been tested on mouse samples?
Verified customer
Asked: 2022-04-07
Answer
Anti-CLPX Antibody Picoband™ (Monoclonal, 11C11) (M00978) is suitable for testing human samples only.
Boster Scientific Support
Answered: 2022-04-07
Question
We are currently using anti-CLPX antibody (monoclonal, 11C11) M00978 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
The anti-CLPX antibody (monoclonal, 11C11) (M00978) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-02
