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Product Info Summary
| SKU: | A02756-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Death-associated protein 1 DAP Antibody
SKU/Catalog Number
A02756-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Death-associated protein 1 DAP Antibody (Catalog # A02756-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
DAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Death-associated protein 1 DAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02756-1)
Host
Rabbit
Contents
DAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP. The immunogen is located within the last 50 amino acids of DAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
DAP antibody is human, mouse and rat reactive.
Reactive Species
A02756-1 is reactive to DAP in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
11165 MW
Background of DAP1
The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02756-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of DAP in human small intestine tissue lysate with DAP antibody at 1 μg/mL.
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Immunohistochemistry of DAP in human small intestine tissue with DAP antibody at 5 μg/ml.
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Immunofluorescence of DAP in human small intestine tissue with DAP antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For DAP (Source: Uniprot.org, NCBI)
Gene Name
DAP
Full Name
Death-associated protein 1
Weight
11165 MW
Alternative Names
DAP1, Death-associated protein 1, DAP-1 DAP death associated protein death-associated protein 1|DAP-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DAP, check out the DAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Death-associated protein 1 DAP Antibody (A02756-1)
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