Click image to see more details
Product Info Summary
| SKU: | EK1463 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Rat MMP-9 ELISA Kit PicoKine®
SKU/Catalog Number
EK1463
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat MMP-9 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Mmp9 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat MMP-9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1463)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L35-P708
Sensitivity
<15 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1463 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1463 is reactive to Mmp9 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1463 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-9
Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1463-CAP | Anti-Rat Mmp9 Pre-coated 96-well strip microplate | 1 |
| EK1463-ST | Rat Mmp9 Standard | 2 vials, 20 ng/tube |
| EK1463-DA | Rat Mmp9 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.047 | 0.115 | 0.195 | 0.321 | 0.615 | 1.001 | 1.635 | 2.065 |
Data Example Images
Click image to see more details
Rat MMP-9 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-9, Dilution ratio of 1:5, concentration in serum and plasma is around 20 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat MMP-9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 429 | 2362 | 8880 | 450 | 2199 | 8392 |
| Standard deviation | 29.17 | 153.53 | 594.96 | 33.75 | 167.12 | 646.18 |
| CV (%) | 6.8% | 6.5% | 6.7% | 7.5% | 7.6% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-9 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 429 | 457 | 420 | 449 | 438 | 14.87 | 3.3% |
| Sample 2 | 2362 | 2453 | 2332 | 2307 | 2363 | 55.22 | 2.3% |
| Sample 3 | 8880 | 10067 | 9013 | 9434 | 9348 | 462.49 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For Mmp9 (Source: Uniprot.Org, NCBI)
Gene Name
Mmp9
Full Name
Matrix metalloproteinase-9
Weight
78611 MW
Superfamily
peptidase M10A family
Alternative Names
Matrix metalloproteinase-9;MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;Mmp9; MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Mmp9, check out the Mmp9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mmp9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat MMP-9 ELISA Kit PicoKine® (EK1463)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Rat MMP-9 ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Rat MMP-9 ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Rat MMP-9 ELISA Kit PicoKine®
Question
Q: Can MMP-9 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Singh
Verified customer
Asked: 2020-03-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-03-01
Question
I wanted to know that MMP-9 is your Rat MMP-9 PicoKine ELISA specific for. The website states natural and recombinant MMP-9 but is it the pro, inactive, or TIMP-complexed form of MMP-9???
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
EK1463 recognizes total form of MMP-9, including
Boster Scientific Support
Answered: 2020-02-21
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Walker
Verified customer
Asked: 2019-11-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-11-24
Question
Q: if the enzyme conjugated MMP-9 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-9 antigen?
Verified Customer
Verified customer
Asked: 2019-02-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-17
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
K. Yang
Verified customer
Asked: 2019-01-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-01-25
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis MMP-9 using the EK1463 Rat MMP-9 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-12-02
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2017-12-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
K. Varma
Verified customer
Asked: 2017-05-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-05-30
Question
Q: how can I thaw whole blood sample for MMP-9 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-08-22
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-9 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-08-22
Question
What form of MMP-9 does this kit recognize?
Verified Customer
Verified customer
Asked: 2013-03-27
Answer
EK1463 recognizes total form of MMP-9, including the pro, inactive, or TIMP-complexed form of MMP-9.
Boster Scientific Support
Answered: 2013-03-27
