This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Death-associated protein 1.
.
Human | |
---|---|
Gene Name: | DAP |
Uniprot: | P51397 |
Entrez: | 1611 |
Belongs to: |
---|
No superfamily |
DAP1; DAP-1; death-associated protein 1; death-associated protein; MGC99796
Mass (kDA):
11.165 kDA
Human | |
---|---|
Location: | 5p15.2 |
Sequence: | 5; NC_000005.10 (10679230..10761234, complement) |
There are a number of reasons to optimize your experiments using Boster Bio. Here are some tips and techniques to optimize your experiments using Boster Bio. If you have any questions or concerns, contact Boster Bio to learn more about optimization. You can also refer to our online guides for specific Boster Bio applications. Hopefully, this information will help you choose the best method for your needs. However, you should consider the following factors before starting your experiment.
The DAP marker was developed in 1993 by Steven Boster, who earned the nickname "he who converts science in the lavatory." His research has paved the way for the development of ELISA kits and hundreds of primary antibodies. By the late 1990s, Boster had become the largest catalog antibody company in China. He also created his own ELISA platform, PicoKine(tm). He also patented the method of enzymatic amplification and developed proprietary trade secrets.
The technique of Western Blotting was first described in 1979 by Towbin et al. The procedure can identify single proteins, protein modifications, and the presence or absence of post-translational modifications. It is also used to quantitatively compare the levels of protein in different samples. While the method is relatively simple, there are some common problems. Unusual bands with weak signals are examples of common problems. High background levels in the blot are another problem. Lastly, blotting methods may fail to detect the protein at all.
Using the right protein marker is essential to obtaining accurate results. While the DAP marker can be purchased online, some manufacturers are very specific in the protein's molecular weight, making it hard to use in a particular experiment. To prevent signal saturation and bloating, the protein must be quantified before preparing it for a Western Blot. For quantitative Westerns, the protein concentration should be at least 20 ng.
The Western Blotting Method uses two main types of samples: tissue and cell lysates. The first is a protein sample, while the second is a soluble cell lysate. Tissue samples are typically more complex and show more structure. A protein ladder is necessary to ensure proper Western transfer efficiency on membranes. This marker is usually provided in gel loading buffer and does not require a reducing agent.
WESTERNVIEW(r) Detection Kits are an excellent choice when performing a Western blot. The kit can reveal bands on a transfer membrane in real-time. Unlike the conventional method of visualization, a WESTERNVIEW(r) Detection Kit is a convenient solution. The WESTERNVIEW(r) Detection Kit includes all the reagents you need to perform your experiment.
The DAP marker can be used to identify proteins that contain the DAP receptor. A primary antibody recognizes the target protein and a secondary antibody provides a means of detection. It can be tagged with an enzyme or fluorescent tags. Once the label is applied, dark regions of the blot correspond to the protein bands of interest. Enhanced chemiluminescence is an excellent alternative to radioactive detection.
The DAP marker is a fluorescent antibody that can be used for many different applications. The dye can be used to detect the presence of DAP in a cell culture, tissue, or tissue-culture medium. Several other applications can also be performed with this dye, including analyzing protein levels and measuring protein concentrations. The article was written by Saheli Sadanand, the primary editor of Nature Medicine. She oversaw the editorial process and peer-review processes.
Validation with the DAP Marker has been conducted to evaluate the performance of whole-cell indirect LiaX ELISA as a surrogate marker of DAP-mediated cell membrane response in Efm strains. DAP-S and DAP-R reference strains are color-coded according to LiaFSR mutations. The dotted line represents an example cutoff value for DAP-S/R for this assay. The coefficient of variation is 15%.
PMID: 7828849 by Deiss L.P., et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death.
PMID: 16602700 by Zougman A., et al. Beyond linker histones and high mobility group proteins: global profiling of perchloric acid soluble proteins.