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Product Info Summary
| SKU: | M02448 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-DDX4 Monoclonal Antibody
SKU/Catalog Number
M02448
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DDX4 Monoclonal Antibody catalog # M02448. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02448)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2F9H5
Isotype
IgG
Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02448 is reactive to DDX4 in Human
Reconstitution
Calculated molecular weight
79308 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02448 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Figure 4. Flow Cytometry validation of DDX4 using Anti-DDX4 Monoclonal Antibody (M02448).
Flow cytometric (FCM) analysis of MSCS cells using DDX4 Monoclonal Antibody (green) and negative control (purple).
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Figure 3. Immunofluorescent staining data of DDX4 using Anti-DDX4 Monoclonal Antibody (M02448).
Immunofluorescence (IF) analysis of MSCs(left) and NTERA-2 (right) cells using DDX4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
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Figure 2. Immunohistochemistry validation of DDX4 using Anti-DDX4 Monoclonal Antibody (M02448).
Immunohistochemistry (IHC) analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B)
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Figure 1. Western blotting validation for Anti-DDX4 Monoclonal Antibody M02448
Western Blot (WB) analysis using DDX4 Monoclonal Antibody against DDX4-hIgGFc transfected HEK293 cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DDX4 (Source: Uniprot.org, NCBI)
Gene Name
DDX4
Full Name
ATP-dependent RNA helicase DDX4
Weight
79308 MW
Superfamily
DEAD box helicase family
Alternative Names
Probable ATP-dependent RNA helicase DDX4;3.6.4.13 Ddx4|AV206478, Mv, Mvh, VASA|DEAD box helicase 4|ATP-dependent RNA helicase DDX4|D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 4|DEAD (Asp-Glu-Ala-Asp) box polypeptide 4|DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 4|DEAD box polypeptide 4|DEAD box protein 4|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4|mvh / mvasa|probable ATP-dependent RNA helicase DDX4|vasa homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDX4, check out the DDX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DDX4 Monoclonal Antibody (M02448)
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No publications found for M02448
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