Anti-DCAMKL3 DCLK3 Antibody

DCLK3 antibody

Boster Bio Anti-DCAMKL3 DCLK3 Antibody catalog # A15882. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A15882
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-DCAMKL3 DCLK3 Antibody

View all DCLK3 Antibodies

SKU/Catalog Number

A15882

Size

100μl

Form

Liquid

Description

Boster Bio Anti-DCAMKL3 DCLK3 Antibody catalog # A15882. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DCAMKL3 DCLK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15882)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human DCAMKL3. at AA rangle: 260-340

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A15882 is reactive to DCLK3 in Human

Applications

A15882 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

73814 MW

Background of DCLK3

BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For DCLK3 (Source: Uniprot.org, NCBI)

Gene Name

DCLK3

Full Name

Serine/threonine-protein kinase DCLK3

Weight

73814 MW

Superfamily

protein kinase superfamily

Alternative Names

CLICK-I; DCAMKL3; DCDC3CCLR; doublecortin and CaM kinase-like 3; Doublecortin domain-containing protein 3C; Doublecortin-like and CAM kinase-like 3; doublecortin-like kinase 3EC 2.7.11.1; EC 2.7.11; II-related; KIAA1765DCK3; serine/threonine-protein kinase DCLK3 DCLK3 CLR, DCAMKL3, DCDC3C, DCK3 doublecortin like kinase 3 serine/threonine-protein kinase DCLK3|CLICK-I,II-related|doublecortin and CaM kinase-like 3|doublecortin domain-containing protein 3C|doublecortin-like and CAM kinase-like 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DCLK3, check out the DCLK3 Infographic

DCLK3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DCLK3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-DCAMKL3 DCLK3 Antibody

Question

See below the WB image, lot number and protocol we used for tibial nerve using anti-DCAMKL3 antibody A15882. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-20

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-20

Question

I have a question about product A15882, anti-DCAMKL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15882 anti-DCAMKL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-03

Question

We are currently using anti-DCAMKL3 antibody A15882 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-30

Answer

The anti-DCAMKL3 antibody (A15882) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-30

Question

I was wanting to use your anti-DCAMKL3 antibody for WB for human tibial nerve on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tibial nerve identification?

Verified Customer

Verified customer

Asked: 2018-08-24

Answer

You can see on the product datasheet, A15882 anti-DCAMKL3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tibial nerve in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-24

Question

Would anti-DCAMKL3 antibody A15882 work on pig WB with tibial nerve?

Verified Customer

Verified customer

Asked: 2018-05-31

Answer

Our lab technicians have not validated anti-DCAMKL3 antibody A15882 on pig. You can run a BLAST between pig and the immunogen sequence of anti-DCAMKL3 antibody A15882 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig tibial nerve in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-31

Question

Does A15882 anti-DCAMKL3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-31

Answer

As indicated on the product datasheet, A15882 anti-DCAMKL3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-31

Order DetailsPrice
A15882

100uL

$399

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A15882
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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