Anti-BRN3A/POU4F1 Antibody Picoband®

Product Name

Anti-BRN3A/POU4F1 Antibody Picoband®

View all BRN3A Antibodies

SKU/Catalog Number

A05403-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BRN3A/POU4F1 Antibody Picoband® catalog # A05403-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BRN3A/POU4F1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05403-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human BRN3A recombinant protein (Position: R311-Y419).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05403-1 is reactive to POU4F1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

45 kDa

Calculated molecular weight

39411 MW

Background of BRN3A

POU domain, class 4, transcription factor 1 (POU4F1), also known as brain-specific homeobox/POU domain protein 3A (BRN3A), homeobox/POU domain protein RDC-1 or Oct-T1, is a protein that in humans is encoded by the POU4F1 gene. It is mapped to 13q31.1. This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05403-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human placenta tissue, human CACO-2 whole cell, human A549 whole cell, human Hela whole cell

Validation Images & Assay Conditions

Gene/Protein Information For POU4F1 (Source: Uniprot.org, NCBI)

Alternative Names

POU domain, class 4, transcription factor 1 POU4F1 BRN3A, Oct-T1, RDC-1, brn-3A POU class 4 homeobox 1 POU domain, class 4, transcription factor 1|brain-specific homeobox/POU domain protein 3A|homeobox/POU domain protein RDC-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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