Product Info Summary
SKU: | A02439-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GPNMB/Gpnmb Picoband® Antibody
View all Osteoactivin/GPNMB Antibodies
SKU/Catalog Number
A02439-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GPNMB/Gpnmb Picoband® Antibody catalog # A02439-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPNMB/Gpnmb Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02439-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02439-1 is reactive to Gpnmb in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
64 kDa
Calculated molecular weight
38779 MW
Background of Osteoactivin/GPNMB
Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02439-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: rat liver tissue, mouse liver tissue, mouse kidney tissue
IHC: human liver cancer Heat mediated antigen retrieval was performed in EDTA buffer (pH80, epitope retrieval solution) The tissue, mouse brain tissue
ICC/IF: A431 cell
FCM: U87 cell, HEPA1-6 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Gpnmb using anti-Gpnmb antibody (A02439-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gpnmb antigen affinity purified polyclonal antibody (Catalog # A02439-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gpnmb at approximately 64KD. The expected band size for Gpnmb is at 64KD.
Click image to see more details
Figure 2. IHC analysis of Gpnmb using anti-Gpnmb antibody (A02439-1).
Gpnmb was detected in paraffin-embedded section of human liver cancer. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Gpnmb using anti-Gpnmb antibody (A02439-1).
Gpnmb was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of Gpnmb using anti-Gpnmb antibody (A02439-1).
Gpnmb was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-Gpnmb Antibody (A02439-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of U87 cells using anti-Gpnmb antibody (A02439-1).
Overlay histogram showing U87 cells stained with A02439-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gpnmb Antibody (A02439-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Click image to see more details
Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti-Gpnmb antibody (A02439-1).
Overlay histogram showing HEPA1-6 cells stained with A02439-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gpnmb Antibody (A02439-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Gpnmb (Source: Uniprot.org, NCBI)
Gene Name
Gpnmb
Full Name
Transmembrane glycoprotein NMB
Weight
38779 MW
Superfamily
PMEL/NMB family
Alternative Names
Transmembrane glycoprotein NMB; DC-HIL; Dendritic cell-associated transmembrane protein; Osteoactivin; Gpnmb; Dchil; Hgfin;;Nmb GPNMB HGFIN, NMB, PLCA3 glycoprotein nmb transmembrane glycoprotein NMB|glycoprotein (transmembrane) nmb|glycoprotein nmb-like protein|glycoprotein nonmetastatic melanoma protein B|hematopoietic growth factor inducible neurokinin-1 type|osteoactivin|transmembrane glycoprotein HGFIN
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Gpnmb, check out the Gpnmb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Gpnmb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPNMB/Gpnmb Picoband® Antibody (A02439-1)
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Customer Q&As
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3 Customer Q&As for Anti-GPNMB/Gpnmb Picoband® Antibody
Question
What is the Concentration of sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances) if it is contained in the product?
Verified customer
Asked: 2022-09-02
Answer
A02439-1 contains 0.02% sodium azide.
Boster Scientific Support
Answered: 2022-09-02
Question
May I know whether the product contain the protein derive from Baculovirus expression system?
Verified customer
Asked: 2022-09-02
Answer
It doesn't contain any protein derive from Baculovirus expression system.
Boster Scientific Support
Answered: 2022-09-02
Question
Please let us know if the following products are purified by affinity column.
Verified customer
Asked: 2022-09-02
Answer
It is antigen affinity purified.
Boster Scientific Support
Answered: 2022-09-02