Anti-GPNMB/Gpnmb Picoband® Antibody

Osteoactivin/GPNMB antibody

Boster Bio Anti-GPNMB/Gpnmb Picoband® Antibody catalog # A02439-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A02439-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-GPNMB/Gpnmb Picoband® Antibody

View all Osteoactivin/GPNMB Antibodies

SKU/Catalog Number

A02439-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GPNMB/Gpnmb Picoband® Antibody catalog # A02439-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPNMB/Gpnmb Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02439-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02439-1 is reactive to Gpnmb in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

64 kDa

Calculated molecular weight

38779 MW

Background of Osteoactivin/GPNMB

Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02439-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: rat liver tissue, mouse liver tissue, mouse kidney tissue
IHC: human liver cancer Heat mediated antigen retrieval was performed in EDTA buffer (pH80, epitope retrieval solution) The tissue, mouse brain tissue
ICC/IF: A431 cell
FCM: U87 cell, HEPA1-6 cell

Validation Images & Assay Conditions

Gene/Protein Information For Gpnmb (Source: Uniprot.org, NCBI)

Gene Name

Gpnmb

Full Name

Transmembrane glycoprotein NMB

Weight

38779 MW

Superfamily

PMEL/NMB family

Alternative Names

Transmembrane glycoprotein NMB; DC-HIL; Dendritic cell-associated transmembrane protein; Osteoactivin; Gpnmb; Dchil; Hgfin;;Nmb GPNMB HGFIN, NMB, PLCA3 glycoprotein nmb transmembrane glycoprotein NMB|glycoprotein (transmembrane) nmb|glycoprotein nmb-like protein|glycoprotein nonmetastatic melanoma protein B|hematopoietic growth factor inducible neurokinin-1 type|osteoactivin|transmembrane glycoprotein HGFIN

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Gpnmb, check out the Gpnmb Infographic

Gpnmb infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Gpnmb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-GPNMB/Gpnmb Picoband® Antibody

Question

What is the Concentration of sodium azide or thimerosal and 2-mercaptoethanol(or other toxic substances) if it is contained in the product?

Verified customer

Asked: 2022-09-02

Answer

A02439-1 contains 0.02% sodium azide.

Boster Scientific Support

Answered: 2022-09-02

Question

May I know whether the product contain the protein derive from Baculovirus expression system?

Verified customer

Asked: 2022-09-02

Answer

It doesn't contain any protein derive from Baculovirus expression system.

Boster Scientific Support

Answered: 2022-09-02

Question

Please let us know if the following products are purified by affinity column.

Verified customer

Asked: 2022-09-02

Answer

It is antigen affinity purified.

Boster Scientific Support

Answered: 2022-09-02

Order DetailsPrice
A02439-1

100μg

$370
A02439-1-10ug

10μg sample (liquid)

$99
A02439-1-Biotin

100 μg Biotin conjugated

$570
A02439-1-Cy3

100 μg Cy3 conjugated

$570
A02439-1-Dylight488

100 μg Dylight488 conjugated

$570
A02439-1-Dylight550

100 μg Dylight550 conjugated

$570
A02439-1-Dylight594

100 μg Dylight594 conjugated

$570
A02439-1-FITC

100 μg FITC conjugated

$570
A02439-1-HRP

100 μg HRP conjugated

$570
A02439-1-APC

100 μg APC conjugated

$670
A02439-1-PE

100 μg PE conjugated

$670
A02439-1-iFluor647

100 μg iFluor647 conjugated

$670
A02439-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A02439-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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