Product Info Summary
SKU: | P03848 |
---|---|
Size: | 100ug |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Phospho-ASAP1 Y782 Antibody
SKU/Catalog Number
P03848
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Phospho-ASAP1 Y782 Antibody (Catalog # P03848). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Phospho-ASAP1 Y782 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03848)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03848 is reactive to ASAP1 in Human, Mouse
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
127421 MW
Background of Asap1
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes. Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03848 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:4,000 - 1:16,000
IHC: 20-40 µg/ml
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Phosphorylated ASAP1 was detected in immunocytochemical sections of mouse NIH3T3 cells transfected with activated Src using rabbit anti-ASAP1 pY782 Antigen Affinity purified polyclonal antibody (Catalog # P03848). Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.
Click image to see more details
ASAP1 was detected in paraffin-embedded sections of epidermal keratinocytes in human skin using rabbit anti-ASAP1 pY782 Antigen Affinity purified polyclonal antibody (Catalog # P03848) at 20 μg/mL. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.
Protein Target Info & Infographic
Gene/Protein Information For ASAP1 (Source: Uniprot.org, NCBI)
Gene Name
ASAP1
Full Name
Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1
Weight
127421 MW
Alternative Names
ASAP1 pY782, ASAP-1, ASAP 1, Development and differentiation enhancing factor 1, 130 kDa phosphatidylinositol 4 5 biphosphate dependent ARF1 GTPase activating protein, ADP ribosylation factor directed GTPase activating protein 1, AMAP 1 Asap1|AV239055, DEF-1, Ddef1, PAP, mKIAA1249, s19|ArfGAP with SH3 domain, ankyrin repeat and PH domain1|arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1|130 kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein|130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein|ADP-ribosylation factor-directed GTPase-activating protein 1|ARF GTPase-activating protein 1|PIP2-dependent ARF1 GAP|development and differentiation-enhancing factor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASAP1, check out the ASAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-ASAP1 Y782 Antibody (P03848)
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-ASAP1 Y782 Antibody
Question
Can you help my question with product P03848, anti-Phospho-ASAP1 Y782 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P03848 anti-Phospho-ASAP1 Y782 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-19
Question
We are currently using anti-Phospho-ASAP1 Y782 antibody P03848 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-Phospho-ASAP1 Y782 antibody (P03848) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-Phospho-ASAP1 Y782 antibody P03848. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-18
Question
I see that the anti-Phospho-ASAP1 Y782 antibody P03848 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-04