Product Info Summary
SKU: | A05265 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-E2F-5 Antibody
SKU/Catalog Number
A05265
Size
100ul
Form
Liquid
Description
Boster Bio Anti-E2F-5 Antibody catalog # A05265. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E2F-5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05265)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human E2F-5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05265 is reactive to E2F5 in Human, Mouse, Rat
Applications
A05265 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
37610 MW
Background of E2F5
Necessary for centrosome duplication. Acts as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, 2 molecules involved in centriole formation. Also plays a key role in deuterosome-mediated centriole amplification in multiciliated that can generate more than 100 centrioles. Overexpression of CEP152 can drive amplification of centrioles.
Nagase T., DNA Res. 5:355-364(1998).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Andersen J.S., Nature 426:570-574(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:20000
Validation Images & Assay Conditions
![a05265 anti e2f 5 antibody wb testing 1 a05265 anti e2f 5 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a05265-anti-e2f-5-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-E2F-5 Antibody A05265
Western Blot (WB) analysis of HeLa cells using E2F-5 polyclonal antibody.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For E2F5 (Source: Uniprot.org, NCBI)
Gene Name
E2F5
Full Name
Transcription factor E2F5
Weight
37610 MW
Superfamily
E2F/DP family
Alternative Names
E2F transcription factor 5, p130-binding; E2F-5; transcription factor E2F5 E2F5 E2F-5 E2F transcription factor 5 transcription factor E2F5|E2F transcription factor 5, p130-binding
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on E2F5, check out the E2F5 Infographic
![E2F5 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for E2F5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E2F-5 Antibody (A05265)
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No publications found for A05265
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