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Product Info Summary
| SKU: | M03679-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-MAML2 Antibody (Center)
SKU/Catalog Number
M03679-1
Size
80 µl
Description
Boster Bio Anti-MAML2 Antibody (Center) (Catalog # M03679-1). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MAML2 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03679-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MAML2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 780-814 amino acids from the Central region of human MAML2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03679-1 is reactive to MAML2 in Human
Applications
M03679-1 is guaranteed for IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
125197 Da
Background of MAML2
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Potentiates activation by NOTCH3 and NOTCH4 more efficiently than MAML1 or MAML3.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-MAML2 Antibody (Center) at 1:2000 dilution
Lane 1: Hela whole cell lysates
Lane 2: SH-SY5Y whole cell lysates
Lane 3: MCF-7 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 125 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M03679-1 staining MAML2 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For MAML2 (Source: Uniprot.org, NCBI)
Gene Name
MAML2
Full Name
Mastermind-like protein 2
Weight
125197 Da
Superfamily
mastermind family
Alternative Names
DKFZp686N0150; KIAA1819MGC176701; MAM2; mam-2; MAM-3; MAM3MLL-MAML2; mastermind (Drosophila)-like 2; mastermind-like 2 (Drosophila); mastermind-like protein 2 MAML2 MAM-3, MAM2, MAM3, MLL-MAML2 mastermind like transcriptional coactivator 2 mastermind-like protein 2|mam-2|mastermind-like 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAML2, check out the MAML2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAML2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAML2 Antibody (Center) (M03679-1)
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