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Product Info Summary
| SKU: | M00398-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 5 (C-term) KRT5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00398-2
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 5 (C-term) KRT5 Rabbit Monoclonal Antibody catalog # M00398-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 5 (C-term) KRT5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00398-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AGD-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 5 (C-term)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00398-2 is reactive to KRT5 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
62378 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00398-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: A431 cell
IHC: human urithelial carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 5 expression in A431 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human urithelial carcinoma, using Cytokeratin 5 (C-term) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For KRT5 (Source: Uniprot.org, NCBI)
Gene Name
KRT5
Full Name
Keratin, type II cytoskeletal 5
Weight
62378 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type II cytoskeletal 5;58 kDa cytokeratin;Cytokeratin-5;CK-5;Keratin-5;K5;Type-II keratin Kb5;KRT5; KRT5 CK5, DDD, DDD1, EBS2, K5A, KRT5 keratin 5 keratin, type II cytoskeletal 5|58 kda cytokeratin|CK-5|cytokeratin-5|epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types|keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)|keratin 5, type II|type-II keratin Kb5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT5, check out the KRT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 5 (C-term) KRT5 Rabbit Monoclonal Antibody (M00398-2)
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No publications found for M00398-2
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Cytokeratin 5 (C-term) KRT5 Rabbit Monoclonal Antibody
Question
We have observed staining in human lymphoblast. Do you have any suggestions? Is anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody supposed to stain lymphoblast positively?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
According to literature lymphoblast does express KRT5. According to Uniprot.org, KRT5 is expressed in ectocervix, brain pancreas, lymphoblast, liver, among other tissues. Regarding which tissues have KRT5 expression, here are a few articles citing expression in various tissues:
Brain, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2019-12-09
Question
I see that the anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoblast using anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2. Let me know if you need anything else.
D. Parker
Verified customer
Asked: 2019-11-11
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
My lab would like to test anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 on human lymphoblast for research purposes, then I may be interested in using anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Yang
Verified customer
Asked: 2019-10-08
Answer
The products we sell, including anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-08
Question
Does M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
It shows on the product datasheet, M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-13
Question
My lab would like using your anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody for keratinization studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
We appreciate your inquiry. This M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody is tested on a431 cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-18
Question
Our lab used your anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody for WB on liver a few months ago. I am using human, and We want to use the antibody for IF next. We want examining liver as well as lymphoblast in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
I have checked the website and datasheets of our anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody and I see that M00398-2 has been validated on human in both WB and IF. Thus M00398-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-26
Question
I was wanting to use your anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody for WB for human lymphoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphoblast identification?
Verified Customer
Verified customer
Asked: 2018-06-01
Answer
You can see on the product datasheet, M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human lymphoblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-01
Question
Here is the WB image, lot number and protocol we used for lymphoblast using anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-20
Question
Is there a BSA free version of anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 available?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-12-14
Question
Is this M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody reactive to the isotypes of KRT5?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
The immunogen of M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody is A synthesized peptide derived from human Cytokeratin 5 (C-term). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-13
Question
We are currently using anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
R. Jones
Verified customer
Asked: 2017-07-10
Answer
The anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody (M00398-2) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-10
Question
Is a blocking peptide available for product anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody (M00398-2)?
Verified Customer
Verified customer
Asked: 2017-05-24
Answer
We do provide the blocking peptide for product anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody (M00398-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-05-24
Question
Does anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 work for WB with lymphoblast?
L. Mitchell
Verified customer
Asked: 2016-10-05
Answer
According to the expression profile of lymphoblast, KRT5 is highly expressed in lymphoblast. So, it is likely that anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody M00398-2 will work for WB with lymphoblast.
Boster Scientific Support
Answered: 2016-10-05
Question
I have a question about product M00398-2, anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Wu
Verified customer
Asked: 2016-08-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00398-2 anti-Cytokeratin 5 (C-term) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-08-18
