Product Info Summary
SKU: | P30451 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody
View all MKP-1/DUSP1 Antibodies
SKU/Catalog Number
P30451
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody (Catalog# P30451). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30451)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human MKP-1/2 around the phosphorylation site of Ser296/318. Immunogen sequence location: 261-310
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P30451 is reactive to DUSP1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
39298 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P30451 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from K562 cells treated with heat shock , using MKP-1/2 (Phospho-Ser296/318) Antibody. The lane on the right is blocked with the phospho peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MKP-1/2 (Phospho-Ser296/318) Antibody. The picture on the right is blocked with the phospho peptide.
Protein Target Info & Infographic
Gene/Protein Information For DUSP1 (Source: Uniprot.org, NCBI)
Gene Name
DUSP1
Full Name
Dual specificity protein phosphatase 1
Weight
39298 MW
Superfamily
protein-tyrosine phosphatase family
Alternative Names
DUSP1;CL100;MKP1;PTPN10;VH1;Dual specificity protein phosphatase 1;Dual specificity protein phosphatase hVH1;Mitogen-activated protein kinase phosphatase 1;MAP kinase phosphatase 1;MKP-1;Protein-tyrosine phosphatase CL100;DUSP4; DUSP1 CL100, HVH1, MKP-1, MKP1, PTPN10 dual specificity phosphatase 1 dual specificity protein phosphatase 1|CL 100|MAP kinase phosphatase 1|dual specificity protein phosphatase hVH1|mitogen-activated protein kinase phosphatase 1|protein-tyrosine phosphatase CL100|serine/threonine specific protein phosphatase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DUSP1, check out the DUSP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DUSP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody (P30451)
Hello CJ!
No publications found for P30451
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-Phospho MKP-1/2 (Ser296/318) DUSP1 Antibody
Question
Is a blocking peptide available for product anti-Phospho MKP-1/2 (Ser296/318) antibody (P30451)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
We do provide the blocking peptide for product anti-Phospho MKP-1/2 (Ser296/318) antibody (P30451). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-23
Question
We are currently using anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?
Z. Thomas
Verified customer
Asked: 2019-12-16
Answer
The anti-Phospho MKP-1/2 (Ser296/318) antibody (P30451) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
Will anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 work on goat IHC with foreskin?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
Our lab technicians have not validated anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat foreskin in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-02
Question
We need to test anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 on mouse brain for research purposes, then I may be interested in using anti-Phospho MKP-1/2 (Ser296/318) antibody P30451 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
The products we sell, including anti-Phospho MKP-1/2 (Ser296/318) antibody P30451, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-14
Question
Here is the WB image, lot number and protocol we used for brain using anti-Phospho MKP-1/2 (Ser296/318) antibody P30451. Please let me know if you require anything else.
N. Krishna
Verified customer
Asked: 2015-10-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-13