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Product Info Summary
| SKU: | M00398 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody
View all KRT5 & KRT8 Antibodies
SKU/Catalog Number
M00398
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody (Catalog # M00398). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Pig, Mouse, Rat, Hamster, Cow, Dog, Sheep, Ferret.
Storage & Handling
Store at 2 to 8°C. Antibody is stable for 24 months.
Cite This Product
Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00398)
Host
Mouse
Contents
Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format.
Clonality
Monoclonal
Clone Number
Clone: C-50
Isotype
IgG1, kappa
Immunogen
Cytoskeletal preparation from HeLa cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00398 is reactive to KRT5 & KRT8 in Cow, Dog, Ferret, Hamster, Human, Mouse, Pig, Rat, Sheep
Reconstitution
Reconstitute with 1ml water (15 minutes at room temperature).
Calculated molecular weight
62378 MW
Background of KRT5 & KRT8
This monoclonal antibody reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00398 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Anti-Cytokeratin 5/8 Monoclonal Antibody (C-50).
Protein Target Info & Infographic
Gene/Protein Information For KRT5 & KRT8 (Source: Uniprot.org, NCBI)
Gene Name
KRT5 & KRT8
Full Name
Weight
62378 MW
Alternative Names
Keratin, type II cytoskeletal 5;58 kDa cytokeratin;Cytokeratin-5;CK-5;Keratin-5;K5;Type-II keratin Kb5;KRT5;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT5 & KRT8, check out the KRT5 & KRT8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT5 & KRT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody (M00398)
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No publications found for M00398
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Customer Q&As
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2 Customer Q&As for Anti-Cytokeratin 5/8 KRT5 Monoclonal Antibody
Question
We are currently using anti-Cytokeratin 5/8 Monoclonal antibody M00398 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, canine, hamster, human, mouse, pig, rat. Is it true that the antibody can work on zebrafish tissues as well?
N. Brown
Verified customer
Asked: 2020-04-07
Answer
The anti-Cytokeratin 5/8 Monoclonal antibody (M00398) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
Question
We have observed staining in rat ectocervix. Do you have any suggestions? Is anti-Cytokeratin 5/8 Monoclonal antibody supposed to stain ectocervix positively?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
From what I have seen in literature ectocervix does express KRT5. From what I have seen in Uniprot.org, KRT5 is expressed in ectocervix, brain pancreas, lymphoblast, liver, among other tissues. Regarding which tissues have KRT5 expression, here are a few articles citing expression in various tissues:
Brain, and Pancreas, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Boster Scientific Support
Answered: 2020-01-02
