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Product Info Summary
| SKU: | PA1335 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 19/KRT19 Antibody Picoband®
View all Cytokeratin 19 Antibodies
SKU/Catalog Number
PA1335
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytokeratin 19/KRT19 Antibody catalog # PA1335. Tested in IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 19/KRT19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1335)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1335 is reactive to KRT19 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
44 kDa
Calculated molecular weight
44106 MW
Background of Cytokeratin 19
Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1335 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human MCF-7 whole cell, human MDA-MB-453 whole cell, human placenta tissue, human RT4 whole cell, human T-47D whole cell, human A431 whole cell, human HL-60 whole cell
IHC: Human Oesophagus Squama Cancer tissue
IHC-F: human placenta tissue
ICC: MCF-7 cell
Validation Images & Assay Conditions
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Figure 2. IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335).
Cytokeratin 19 was detected in a paraffin-embedded section of Human Oesophagus Squama Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 1. Western blot analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human MDA-MB-453 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: human T-47D whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 19 antigen affinity purified polyclonal antibody (Catalog # PA1335) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for Cytokeratin 19 at approximately 44 kDa. The expected band size for Cytokeratin 19 is at 44 kDa.
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Figure 3. ICC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335).
Cytokeratin 19 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of Cytokeratin 19 using anti-Cytokeratin 19 antibody (PA1335).
Cytokeratin 19 was detected in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytokeratin 19 Antibody (PA1335) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KRT19 (Source: Uniprot.org, NCBI)
Gene Name
KRT19
Full Name
Keratin, type I cytoskeletal 19
Weight
44106 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type I cytoskeletal 19;Cytokeratin-19;CK-19;Keratin-19;K19;KRT19; KRT19 CK19, K19, K1CS keratin 19 keratin, type I cytoskeletal 19|40-kDa keratin intermediate filament|CK-19|cytokeratin 19|keratin 19, type I|keratin, type I, 40-kd
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT19, check out the KRT19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 19/KRT19 Antibody Picoband® (PA1335)
Hello CJ!
PA1335 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu T,Tang C,Chen Y,Yong X,Liu Z,Jiang L,Zeng Q,Tao R.Regulatory effect of 17β-estradiol on the expression of β-defensin-2 and proinflammatory cytokines in human oral epithelial cells.J Oral Pathol Med.2020 Apr;49(4):365-372.doi:10.1111/jop.13016.Epub 2020
Species: Human
PA1335 usage in article: APP:ICC, SAMPLE:HOMECS, DILUTION:1:100
Selective tropism of liver stem cells to hepatocellular carcinoma in vivo.
Quan, J., Du, Q., Hou, Y., Wang, Z., & Zhang, J. (2017). Utilization of E-cadherin by monocytes from tumour cells plays key roles in the progression of bone invasion by oral squamous cell carcinoma. Oncology Reports, 38(2), 850-858. Advance online...
Yin D, Tian L, Ye Y, Li K, Wang J, Cheng P, Chen A, Guo F, Huang H. Int J Mol Med. 2012 Apr;29(4):587-92. Doi: 10.3892/Ijmm.2011.871. Epub 2011 Dec 29. Nanog And ??-Catenin: A New Convergence Point In Epsc Proliferation And Differentiation.
Lu J, Gu Yp, Xu X, Liu Ml, Xie P, Song Hp. World J Gastroenterol. 2005 Jun 14;11(22):3426-30. Adult Islets Cultured In Collagen Gel Transdifferentiate Into Duct-Like Cells.
Li M, Zhang B, Zhang Z, Liu X, Qi X, Zhao J, Jiang Y, Zhai H, Ji Y, Luo D. Biomed Res Int. 2014;2014:981261. Doi: 10.1155/2014/981261. Epub 2014 May 22. Stem Cell-Like Circulating Tumor Cells Indicate Poor Prognosis In Gastric Cancer.
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Customer Q&As
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16 Customer Q&As for Anti-Cytokeratin 19/KRT19 Antibody Picoband®
Question
I see that the anti-Cytokeratin 19/KRT19 antibody PA1335 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-14
Question
Will anti-Cytokeratin 19/KRT19 antibody PA1335 work on pig IHC with mammary gland?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
Our lab technicians have not validated anti-Cytokeratin 19/KRT19 antibody PA1335 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Cytokeratin 19/KRT19 antibody PA1335 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig mammary gland in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-11
Question
My question regarding product PA1335, anti-Cytokeratin 19/KRT19 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1335 anti-Cytokeratin 19/KRT19 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-18
Question
Does PA1335 anti-Cytokeratin 19/KRT19 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
As indicated on the product datasheet, PA1335 anti-Cytokeratin 19/KRT19 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-27
Question
I was wanting to use using your anti-Cytokeratin 19/KRT19 antibody for formation of the cornified envelope studies. Has this antibody been tested with western blotting on squama cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
We appreciate your inquiry. This PA1335 anti-Cytokeratin 19/KRT19 antibody is tested on ht1080 cell lysate, colo320 cell lysate, squama cancer tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-17
Question
Is this PA1335 anti-Cytokeratin 19/KRT19 antibody reactive to the isotypes of KRT19?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
The immunogen of PA1335 anti-Cytokeratin 19/KRT19 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Cytokeratin 19(379-392aa YRSLLEGQEDHYNN). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-16
Question
I was wanting to use your anti-Cytokeratin 19/KRT19 antibody for ICC for human lower esophagus mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lower esophagus mucosa identification?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
It shows on the product datasheet, PA1335 anti-Cytokeratin 19/KRT19 antibody has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lower esophagus mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-15
Question
We are currently using anti-Cytokeratin 19/KRT19 antibody PA1335 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
The anti-Cytokeratin 19/KRT19 antibody (PA1335) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-04
Question
you antibody to test anti-Cytokeratin 19/KRT19 antibody PA1335 on human lower esophagus mucosa for research purposes, then I may be interested in using anti-Cytokeratin 19/KRT19 antibody PA1335 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The products we sell, including anti-Cytokeratin 19/KRT19 antibody PA1335, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-05
Question
Do you have a BSA free version of anti-Cytokeratin 19/KRT19 antibody PA1335 available?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cytokeratin 19/KRT19 antibody PA1335 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-04
Question
Will anti-Cytokeratin 19/KRT19 antibody PA1335 work for ICC with lower esophagus mucosa?
J. Yang
Verified customer
Asked: 2018-09-04
Answer
According to the expression profile of lower esophagus mucosa, KRT19 is highly expressed in lower esophagus mucosa. So, it is likely that anti-Cytokeratin 19/KRT19 antibody PA1335 will work for ICC with lower esophagus mucosa.
Boster Scientific Support
Answered: 2018-09-04
Question
We have observed staining in human peripheral blood leukocyte. Any tips? Is anti-Cytokeratin 19/KRT19 antibody supposed to stain peripheral blood leukocyte positively?
C. Kulkarni
Verified customer
Asked: 2018-08-15
Answer
According to literature peripheral blood leukocyte does express KRT19. According to Uniprot.org, KRT19 is expressed in lower esophagus mucosa, placenta, mammary gland, pancreas placenta, peripheral blood leukocyte, keratinocyte, lymph node, cervix carcinoma erythroleukemia, colon carcinoma, among other tissues. Regarding which tissues have KRT19 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Keratinocyte, Pubmed ID: 1286667
Lymph node, Pubmed ID: 10212274
Mammary gland, Pancreas, and Placenta, Pubmed ID: 15489334
Peripheral blood leukocyte, Pubmed ID: 11682035
Placenta, Pubmed ID: 2447559, 2469734, 14702039
Boster Scientific Support
Answered: 2018-08-15
Question
Is a blocking peptide available for product anti-Cytokeratin 19/KRT19 antibody (PA1335)?
A. Johnson
Verified customer
Asked: 2017-03-17
Answer
We do provide the blocking peptide for product anti-Cytokeratin 19/KRT19 antibody (PA1335). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-03-17
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Cytokeratin 19/KRT19 antibody PA1335. Let me know if you need anything else.
R. Miller
Verified customer
Asked: 2016-04-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-20
Question
We bought anti-Cytokeratin 19/KRT19 antibody for WB on lymph node last year. I am using human, and We intend to use the antibody for IHC next. I am looking for examining lymph node as well as lower esophagus mucosa in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
M. Roberts
Verified customer
Asked: 2014-12-10
Answer
I looked at the website and datasheets of our anti-Cytokeratin 19/KRT19 antibody and it appears that PA1335 has been tested on human in both WB and IHC. Thus PA1335 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-12-10
Question
Please see the WB image, lot number and protocol we used for lower esophagus mucosa using anti-Cytokeratin 19/KRT19 antibody PA1335. Please let me know if you require anything else.
F. Baker
Verified customer
Asked: 2014-10-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-28
