Product Info Summary
SKU: | A00924 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Cytochrome c-type Heme Lyase HCCS Antibody
SKU/Catalog Number
A00924
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cytochrome c-type Heme Lyase HCCS Antibody (Catalog # A00924). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome c-type Heme Lyase HCCS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00924)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CCHL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00924 is reactive to HCCS in Human, Mouse
Reconstitution
Calculated molecular weight
30602 MW
Background of HCCS
Links covalently the heme group to the apoprotein of cytochrome c By similarity.
Schaefer L., Genomics 34:166-172(1996).
van den Veyver I.B., Am. J. Med. Genet. 78:179-181(1998).
The MGC Project Team; Genome Res. 14:2121-2127(2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00924 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue using Cytochrome c-type Heme Lyase antibody A00924.
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Western blot analysis of extracts from COS7 cells, using Cytochrome c-type Heme Lyase antibody A00924.
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Immunofluorescence analysis of MCF-7 cells, using Cytochrome c-type Heme Lyase antibody A00924.
Protein Target Info & Infographic
Gene/Protein Information For HCCS (Source: Uniprot.org, NCBI)
Gene Name
HCCS
Full Name
Cytochrome c-type heme lyase
Weight
30602 MW
Superfamily
cytochrome c-type heme lyase family
Alternative Names
Cytochrome c-type heme lyase;CCHL;4.4.1.17;Holocytochrome c-type synthase;HCCS;CCHL; HCCS CCHL, LSDMCA1, MCOPS7, MLS holocytochrome c synthase holocytochrome c-type synthase|cytochrome c heme-lyase|cytochrome c-type heme lyase|holocytochrome-c synthetase|microphthalamia with linear skin defects|microphthalmia with linear skin defects
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HCCS, check out the HCCS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HCCS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome c-type Heme Lyase HCCS Antibody (A00924)
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Customer Q&As
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3 Customer Q&As for Anti-Cytochrome c-type Heme Lyase HCCS Antibody
Question
We are currently using anti-Cytochrome c-type Heme Lyase antibody A00924 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-Cytochrome c-type Heme Lyase antibody (A00924) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
Is a blocking peptide available for product anti-Cytochrome c-type Heme Lyase antibody (A00924)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
We do provide the blocking peptide for product anti-Cytochrome c-type Heme Lyase antibody (A00924). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-03
Question
I see that the anti-Cytochrome c-type Heme Lyase antibody A00924 works with IHC, what is the protocol used to produce the result images on the product page?
Z. Parker
Verified customer
Asked: 2018-05-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-22