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Product Info Summary
| SKU: | A04753-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-AOS1 SAE1 Antibody
View all SUMO Activating Enzyme E1 (SAE1) Antibodies
SKU/Catalog Number
A04753-2
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-AOS1 SAE1 Antibody (Catalog # A04753-2). Tested in WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AOS1 SAE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04753-2)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human AOS1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04753-2 is reactive to SAE1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
38450 MW
Background of SUMO Activating Enzyme E1 (SAE1)
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096.
Walter Makous, Invest. Ophthalmol. Vis. Sci., Sep 2006; 47: 4160 - 4167.
Hsin-Pei Shih, J. Cell Sci., Mar 2002; 115: 1259.
Thomas Buschmann, J. Biol. Chem., Oct 2001; 276: 40389 - 40395.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04753-2 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from 293 cells, using AOS1 antibody A04753-2.
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Western blot analysis of extracts from Colo cells, HepG2 cells and 293 cells using AOS1 antibody A04753-2. The lane on the left is treated with synthesized peptide.
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Immunofluorescence analysis of HuvEc cells, using AOS1 antibody A04753-2.
Protein Target Info & Infographic
Gene/Protein Information For SAE1 (Source: Uniprot.org, NCBI)
Gene Name
SAE1
Full Name
SUMO-activating enzyme subunit 1
Weight
38450 MW
Superfamily
ubiquitin-activating E1 family
Alternative Names
SUMO-activating enzyme subunit 1;Ubiquitin-like 1-activating enzyme E1A;SUMO-activating enzyme subunit 1, N-terminally processed;SAE1;AOS1, SUA1, UBLE1A; SAE1 AOS1, HSPC140, SUA1, UBLE1A SUMO1 activating enzyme subunit 1 SUMO-activating enzyme subunit 1|SUMO-1 activating enzyme E1 N subunit|SUMO-1 activating enzyme subunit 1|activator of SUMO1|sentrin/SUMO-activating protein AOS1|ubiquitin-like 1-activating enzyme E1A|ubiquitin-like protein SUMO-1 activating enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SAE1, check out the SAE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SAE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AOS1 SAE1 Antibody (A04753-2)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-AOS1 SAE1 Antibody
Question
My question regarding product A04753-2, anti-AOS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04753-2 anti-AOS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-20
Question
Is a blocking peptide available for product anti-AOS1 antibody (A04753-2)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We do provide the blocking peptide for product anti-AOS1 antibody (A04753-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-10
Question
We are currently using anti-AOS1 antibody A04753-2 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
The anti-AOS1 antibody (A04753-2) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-24
Question
Would anti-AOS1 antibody A04753-2 work for IF with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to the expression profile of cervix carcinoma, SAE1 is highly expressed in cervix carcinoma. So, it is likely that anti-AOS1 antibody A04753-2 will work for IF with cervix carcinoma.
Boster Scientific Support
Answered: 2019-08-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-AOS1 antibody A04753-2. Let me know if you need anything else.
P. Lewis
Verified customer
Asked: 2019-02-15
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-15
Question
I see that the anti-AOS1 antibody A04753-2 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-04
Question
I was wanting to use your anti-AOS1 antibody for IF for rat cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
You can see on the product datasheet, A04753-2 anti-AOS1 antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-09
Question
Is this A04753-2 anti-AOS1 antibody reactive to the isotypes of SAE1?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
The immunogen of A04753-2 anti-AOS1 antibody is Synthesized peptide derived from Internal of human AOS1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-26
Question
Will A04753-2 anti-AOS1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-09-28
Answer
It shows on the product datasheet, A04753-2 anti-AOS1 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-09-28
Question
I am looking for to test anti-AOS1 antibody A04753-2 on rat cervix carcinoma for research purposes, then I may be interested in using anti-AOS1 antibody A04753-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The products we sell, including anti-AOS1 antibody A04753-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-28
Question
Do you have a BSA free version of anti-AOS1 antibody A04753-2 available?
D. Wu
Verified customer
Asked: 2017-07-19
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-AOS1 antibody A04753-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-19
Question
See below the WB image, lot number and protocol we used for cervix carcinoma using anti-AOS1 antibody A04753-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-11
Question
Would anti-AOS1 antibody A04753-2 work on feline WB with colon?
K. Kulkarni
Verified customer
Asked: 2015-07-10
Answer
Our lab technicians have not tested anti-AOS1 antibody A04753-2 on feline. You can run a BLAST between feline and the immunogen sequence of anti-AOS1 antibody A04753-2 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-10
