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Product Info Summary
| SKU: | PA2274 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MEK2/MAP2K2 Antibody Picoband®
SKU/Catalog Number
PA2274
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEK2/MAP2K2 Antibody catalog # PA2274. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK2/MAP2K2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2274)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MEK2, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2274 is reactive to MAP2K2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
44424 MW
Background of MEK2
Dual specificity mitogen-activated protein kinase kinase 2 (MAP2K2), also called PRKMK2 or MEK2, is an enzyme that in humans is encoded by the MAP2K2 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MAP2K2 is mapped to 19p13.3. This kinase is known to play a critical role in mitogen growth factor signal transduction, and the inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. Recombinant MEK2 and MEK1 both could activate human ERK1 in vitro, and they further characterized biochemically the 2 MAP2Ks. MAP2K2 has been shown to interact with MAPK3 and ARAF.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2274 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Skeletal Muscle Tissue, Rat Kidney Tissue, HELA Whole Cell, COLO320 Whole Cell, PC12 Whole Cell, JURKAT Whole Cell, HT1080 Whole Cell
Validation Images & Assay Conditions
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Anti-MEK2 antibody, PA2274, All Western blotting
All lanes: MAP2K2(PA2274) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug
Lane 2: Rat Kidney Tissue Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Lane 5: PC12 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
Protein Target Info & Infographic
Gene/Protein Information For MAP2K2 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K2
Full Name
Dual specificity mitogen-activated protein kinase kinase 2
Weight
44424 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 2;MAP kinase kinase 2;MAPKK 2;2.7.12.2;ERK activator kinase 2;MAPK/ERK kinase 2;MEK 2;MAP2K2;MEK2, MKK2, PRKMK2; MAP2K2 CFC4, MAPKK2, MEK2, MKK2, PRKMK2 mitogen-activated protein kinase kinase 2 dual specificity mitogen-activated protein kinase kinase 2|ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|mitogen-activated protein kinase kinase 2, p45
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K2, check out the MAP2K2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK2/MAP2K2 Antibody Picoband® (PA2274)
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No publications found for PA2274
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Customer Q&As
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1 Customer Q&As for Anti-MEK2/MAP2K2 Antibody Picoband®
Question
We are currently using anti-MEK2/MAP2K2 antibody PA2274 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
R. Mitchell
Verified customer
Asked: 2014-12-05
Answer
The anti-MEK2/MAP2K2 antibody (PA2274) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-05
