Anti-MEK2/MAP2K2 Antibody Picoband®

Product Name

Anti-MEK2/MAP2K2 Antibody Picoband®

View all MEK2 Antibodies

SKU/Catalog Number

PA2274

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MEK2/MAP2K2 Antibody catalog # PA2274. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MEK2/MAP2K2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2274)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human MEK2, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2274 is reactive to MAP2K2 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50 kDa

Calculated molecular weight

44424 MW

Background of MEK2

Dual specificity mitogen-activated protein kinase kinase 2 (MAP2K2), also called PRKMK2 or MEK2, is an enzyme that in humans is encoded by the MAP2K2 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MAP2K2 is mapped to 19p13.3. This kinase is known to play a critical role in mitogen growth factor signal transduction, and the inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. Recombinant MEK2 and MEK1 both could activate human ERK1 in vitro, and they further characterized biochemically the 2 MAP2Ks. MAP2K2 has been shown to interact with MAPK3 and ARAF.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2274 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

WB: Rat Skeletal Muscle Tissue, Rat Kidney Tissue, HELA Whole Cell, COLO320 Whole Cell, PC12 Whole Cell, JURKAT Whole Cell, HT1080 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For MAP2K2 (Source: Uniprot.org, NCBI)

Alternative Names

Dual specificity mitogen-activated protein kinase kinase 2;MAP kinase kinase 2;MAPKK 2;2.7.12.2;ERK activator kinase 2;MAPK/ERK kinase 2;MEK 2;MAP2K2;MEK2, MKK2, PRKMK2; MAP2K2 CFC4, MAPKK2, MEK2, MKK2, PRKMK2 mitogen-activated protein kinase kinase 2 dual specificity mitogen-activated protein kinase kinase 2|ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|mitogen-activated protein kinase kinase 2, p45

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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