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Product Info Summary
| SKU: | PB10055 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband®
SKU/Catalog Number
PB10055
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® catalog # PB10055. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10055)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CYP3A4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10055 is reactive to CYP3A4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
57343 MW
Background of CYP3A4
Cytochrome P450 3A4 (abbreviated CYP3A4), is an important enzyme in the body, mainly found in the liver and in the intestine. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10055 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CYP3A4 using anti-CYP3A4 antibody (PB10055).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP3A4 antigen affinity purified polyclonal antibody (Catalog # PB10055) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYP3A4 at approximately 57 kDa. The expected band size for CYP3A4 is at 57 kDa.
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Figure 2. IHC analysis of CYP3A4 using anti-CYP3A4 antibody (PB10055).
CYP3A4 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CYP3A4 Antibody (PB10055) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CYP3A4 (Source: Uniprot.org, NCBI)
Gene Name
CYP3A4
Full Name
Cytochrome P450 3A4
Weight
57343 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 3A4;1.14.13.-;1,8-cineole 2-exo-monooxygenase;1.14.13.157;Albendazole monooxygenase;1.14.13.32;Albendazole sulfoxidase;CYPIIIA3;CYPIIIA4;Cholesterol 25-hydroxylase ;1.14.14.1 ;Cytochrome P450 3A3;Cytochrome P450 HLp;Cytochrome P450 NF-25;Cytochrome P450-PCN1;Nifedipine oxidase;Quinine 3-monooxygenase;1.14.13.67;Taurochenodeoxycholate 6-alpha-hydroxylase;1.14.13.97;CYP3A4;CYP3A3; CYP3A4 CP33, CP34, CYP3A, CYP3A3, CYPIIIA3, CYPIIIA4, HLP, NF-25, P450C3, P450PCN1, VDDR3 cytochrome P450 family 3 subfamily A member 4 cytochrome P450 3A4|1,4-cineole 2-exo-monooxygenase|1,8-cineole 2-exo-monooxygenase|P450-III, steroid inducible|albendazole monooxygenase (sulfoxide-forming)|albendazole sulfoxidase|cholesterol 25-hydroxylase|cytochrome P450 3A3|cytochrome P450 HLp|cytochrome P450 NF-25|cytochrome P450, family 3, subfamily A, polypeptide 4|cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3|cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4|cytochrome P450-PCN1|glucocorticoid-inducible P450|nifedipine oxidase|quinine 3-monooxygenase|taurochenodeoxycholate 6-alpha-hydroxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP3A4, check out the CYP3A4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP3A4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband® (PB10055)
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Customer Q&As
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1 Customer Q&As for Anti-Cytochrome P450 3A4/CYP3A4 Antibody Picoband®
Question
We are currently using anti-Cytochrome P450 3A4/CYP3A4 antibody PB10055 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
The anti-Cytochrome P450 3A4/CYP3A4 antibody (PB10055) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
