Product Info Summary
SKU: | A00543T395 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody
SKU/Catalog Number
A00543T395
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody catalog # A00543T395. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00543T395)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Cyclin E1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00543T395 is reactive to CCNE1 in Human, Mouse, Rat
Applications
A00543T395 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
47077 MW
Background of Cyclin E1
Essential for the control of the cell cycle at the G1/S (start) transition.
Charles Spruck, Cancer Res., Jul 2006; 66: 7355 - 7360.
Nathalie Cueille, J. Virol., Sep 1998; 72: 7255 - 7262.
Wentao Deng, J. Virol., Dec 2004; 78: 13954 - 13965.
Tianlin Ma, PNAS, Jan 1999; 96: 382.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
Validation Images & Assay Conditions
![a00543t395 anti cyclin e1 phospho t395 ccne1 antibody ihc testing 2 a00543t395 anti cyclin e1 phospho t395 ccne1 antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a00543t395-anti-cyclin-e1-phospho-t395-ccne1-antibody-ihc-testing-2.jpg)
Click image to see more details
Figure 2. Immunohistochemistry validation of CCNE1 using Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody (A00543T395).
Immunohistochemistry (IHC) analyzes of p-Cyclin E1 (T395) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.For more protocol information of IHC
![a00543t395 anti cyclin e1 phospho t395 ccne1 antibody wb testing 1 a00543t395 anti cyclin e1 phospho t395 ccne1 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a00543t395-anti-cyclin-e1-phospho-t395-ccne1-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody A00543T395
Western blot (WB) analysis of p-Cyclin E1 (T395) pAb at 1:500 dilutionLane1:A549 whole cell lysate(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:C6 whole cell lysate(40ug)
Lane4:BV2 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CCNE1 (Source: Uniprot.org, NCBI)
Gene Name
CCNE1
Full Name
G1/S-specific cyclin-E1
Weight
47077 MW
Superfamily
cyclin family
Alternative Names
CCNE; CCNE1; CCNEcyclin Es; Cyclin E1; cyclin Et; G1/S-specific cyclin-E1 CCNE1 CCNE, pCCNE1 cyclin E1 G1/S-specific cyclin-E1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNE1, check out the CCNE1 Infographic
![CCNE1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody (A00543T395)
Hello CJ!
No publications found for A00543T395
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cyclin E1 (phospho-T395) CCNE1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question