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Product Info Summary
| SKU: | M00295 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-CXCR3 Monoclonal Antibody
SKU/Catalog Number
M00295
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CXCR3 Monoclonal Antibody catalog # M00295. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00295)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACFD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CXCR3 Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC) . Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC) . May play a role in angiogenesis.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00295 is reactive to CXCR3 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
3 kDa
Calculated molecular weight
124319 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00295 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of CXCR3 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CXCR3 (Source: Uniprot.org, NCBI)
Gene Name
CXCR3
Full Name
C-X-C chemokine receptor type 3
Weight
124319 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Proto-oncogene tyrosine-protein kinase receptor Ret;2.7.10.1;Cadherin family member 12;Proto-oncogene c-Ret;Soluble RET kinase fragment;Extracellular cell-membrane anchored RET cadherin 120 kDa fragment;RET;CDHF12, CDHR16, PTC, RET51; CXCR3 CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR C-X-C motif chemokine receptor 3 C-X-C chemokine receptor type 3|G protein-coupled receptor 9|IP-10 receptor|Mig receptor|chemokine (C-X-C motif) receptor 3|chemokine receptor 3|interferon-inducible protein 10 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR3, check out the CXCR3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR3 Monoclonal Antibody (M00295)
Hello CJ!
M00295 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Impact of chemokine receptor CXCR3 on tumor-infiltrating lymphocyte recruitment associated with favorable prognosis in advanced gastric cancer
An alternatively spliced variant of CXCR3 mediates the metastasis of CD133+ liver cancer cells induced by CXCL9
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Customer Q&As
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5 Customer Q&As for Anti-CXCR3 Monoclonal Antibody
Question
What is the immunogen region of the peptide for M00295?
Verified customer
Asked: 2022-03-08
Answer
The immunogen sequence for the Anti-CXCR3 Monoclonal Antibody (M00295) is GALARNCGRESRVDV, aa284-298 (extracellular).
Boster Scientific Support
Answered: 2022-03-09
Question
We have been able to see staining in human brain. Any tips? Is anti-CXCR3 Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2018-11-09
Answer
According to literature brain does express CXCR3. According to Uniprot.org, CXCR3 is expressed in leukocyte, blood, endothelial cell thymus, colon, brain, lung testis, fetal astrocyte, among other tissues. Regarding which tissues have CXCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9064356
Brain, Lung, and Testis, Pubmed ID: 15489334
Colon, Pubmed ID: 14702039
Endothelial cell, and Thymus, Pubmed ID: 18291705
Fetal astrocyte, Pubmed ID: 9625760
Boster Scientific Support
Answered: 2018-11-09
Question
We are currently using anti-CXCR3 Monoclonal antibody M00295 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
G. Carter
Verified customer
Asked: 2018-10-18
Answer
The anti-CXCR3 Monoclonal antibody (M00295) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-18
Question
We purchased anti-CXCR3 Monoclonal antibody for WB on blood last year. I am using human, and I plan to use the antibody for IF next. We need examining blood as well as leukocyte in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
I looked at the website and datasheets of our anti-CXCR3 Monoclonal antibody and it appears that M00295 has been validated on human in both WB and IF. Thus M00295 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-31
Question
Our lab were satisfied with the WB result of your anti-CXCR3 Monoclonal antibody. However we have seen positive staining in leukocyte isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is CXCR3 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
Based on literature, leukocyte does express CXCR3. Generally CXCR3 expresses in isoform 1: cell membrane, isoform 2: cell membrane. Regarding which tissues have CXCR3 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9064356
Brain, Lung, and Testis, Pubmed ID: 15489334
Colon, Pubmed ID: 14702039
Endothelial cell, and Thymus, Pubmed ID: 18291705
Fetal astrocyte, Pubmed ID: 9625760
Boster Scientific Support
Answered: 2017-08-30
