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Product Info Summary
| SKU: | A01395 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-PLB-T17 Antibody
View all Phospholamban Antibodies
SKU/Catalog Number
A01395
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-PLB-T17 Antibody (Catalog # A01395). Tested in Flow Cytometry, IHC-P, WB application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PLB-T17 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01395)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This PLB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids of human PLB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01395 is reactive to PLN in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
6109 Da
Background of Phospholamban
PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01395 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-PLB Antibody at 1:2000 dilution
Lane 1: human heart lysate
Lane 2: mouse heart lysate
Lane 3: rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 6 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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PLB-T17 Antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLB-T17 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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PLB-T17 Antibody flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For PLN (Source: Uniprot.org, NCBI)
Gene Name
PLN
Full Name
Cardiac phospholamban
Weight
6109 Da
Superfamily
phospholamban family
Alternative Names
Cardiac phospholamban, PLB, PLN, PLB PLN CMD1P, CMH18, PLB phospholamban cardiac phospholamban
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLN, check out the PLN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PLB-T17 Antibody (A01395)
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