Anti-Cul5/Cullin 5 Antibody

Product Name

Anti-Cul5/Cullin 5 Antibody

View all Cullin 5 Antibodies

SKU/Catalog Number

A01925

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Cul5/Cullin 5 Antibody (Catalog # A01925). Tested in IHC, IP, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Cul5/Cullin 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01925)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 1-25 of Human Cul5 (N-terminus) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A01925 is reactive to CUL5 in Human

Applications

A01925 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

90955 MW

Background of Cullin 5

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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Cul5 was detected in paraffin-embedded sections of human kidney tissues using rabbit anti-Cul5 Antiserum, not purified polyclonal antibody (Catalog # A01925) at 1:500. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

Gene/Protein Information For CUL5 (Source: Uniprot.org, NCBI)

Alternative Names

CUL-5; cullin 5; VACM1Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); VACM-1cullin-5; Vasopressin-activated calcium-mobilizing receptor 1; Vasopressin-activated calcium-mobilizing receptor-1 CUL5 CUL-5, VACM-1, VACM1 cullin 5 cullin-5|Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1)|Vasopressin-activated calcium-mobilizing receptor-1|vasopressin-activated calcium-mobilizing receptor 1

For more info on CUL5, check out the CUL5 Infographic

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In this infographic, you will see the following information for CUL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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