Anti-DELTA-4 DLL4 Antibody

Product Name

Anti-DELTA-4 DLL4 Antibody

View all Dll4 Antibodies

SKU/Catalog Number

A00875-2

Size

25uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-DELTA-4 DLL4 Antibody (Catalog # A00875-2). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-DELTA-4 DLL4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00875-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00875-2 is reactive to DLL4 in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL

Observed Molecular Weight

68 kDa

Calculated molecular weight

45983 MW

Background of Dll4

DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. DELTA-4 plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00875-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
IHC: 10 µg/mL to 20 µg/mL
WB: 1:500 - 1:2,500

Validation Images & Assay Conditions

Gene/Protein Information For DLL4 (Source: Uniprot.org, NCBI)

Alternative Names

DELTA4, DELTA-4, delta 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4, notch ligand DLL4 precursor, DELTA 4 Dll4|Delt, Delta4|delta like canonical Notch ligand 4|delta-like protein 4|delta-like 4|drosophila Delta homolog 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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