Cullin-5 Antibodies

Cullin-5 (CUL5)

Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme.

The functional specificity of the E3 ubiquitin-protein ligase complex is based upon the variable substrate recognition component. ECS(SOCS1) appears to direct ubiquitination of JAK2.

Gene Information

Human
Gene Name:	CUL5
Uniprot:	Q93034
Entrez:	8065

Family

Belongs to:
cullin family

Alternative Names

CUL-5; cullin 5; VACM1Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); VACM-1cullin-5; Vasopressin-activated calcium-mobilizing receptor 1; Vasopressin-activated calcium-mobilizing receptor-1

Molecular Weight

Mass (kDA):
90.955 kDA

Genomic Context

Human
Location:	11q22.3
Sequence:	11; NC_000011.10 (108008733..108107766)

Diseases

• Hiv Infections
• Immunologic Deficiency Syndromes
• Neoplasms
• Malignant Neoplasms
• Nervousness
• Tissue Adhesions
• Inflammation
• Acquired Immunodeficiency Syndrome
• Disease Of Adrenal Medulla
• Malignant Neoplasm Of Breast
• Von Hippel-lindau Syndrome
• Leukemia

• Adenovirus Infections
• Hyperplasia
• Mammary Neoplasms
• Cell Transformation, Neoplastic
• Ataxia
• Acute Promyelocytic Leukemia

Interacting Proteins

• ARIH2
• ASB1
• ASB2
• ASB6
• ASB7

• CCNDBP1
• ERBB2
• GOLGA2
• HSPA1A
• RBX1

• RNF7
• SOCS2

Pathways

• Viral Replication
• Cell Cycle
• Cell Growth
• Cell Adhesion
• Localization
• Transport
• Proteolysis
• Cell Proliferation
• Conjugation
• Pathogenesis
• Rna Interference
• Cell Activation
• Reverse Transcription

• Translation
• Regulation Of Cell Cycle
• Dna Replication
• S Phase
• Regulation Of Signaling
• T Cell Activation
• Aging

PTMs

• Ubiquitination
• Phosphorylation

• Sumoylation
• Methylation

Research Areas

• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CUL5

Best Uses Of The CUL5 Marker From Boster Bio

In this article, we will talk about the Anti-Cul5/Cullin 5 Antibody and its application in Data mining and detection. You can use this marker to find and classify species and applications. This article is applicable to all scientists around the world. Read on to learn more. Interested? Start your research now. Here are some of the most popular uses of the CUL5 marker.

Anti-Cul5/Cullin 5 Antibody

The anti-Cul5/Cullin 5 antibodies from Boster Bio react with Human. The antibody is highly specific and is suitable for IHC applications. It is produced from whole rabbit serum and synthetic peptides. It can be stored at -20 degrees Celsius. The Boster Bio anti-Cul5/Cullin 5 antibody is available in a convenient picomand.

The Cullin family comprises a number of proteins. The Cullin 5 antibody from Boster Bio recognizes the protein Cul5. The proteins are part of the SOCS/BC-box/eloBC/cul5/ring E3 ligase complex, which is essential for cell division and cellular metabolism. Moreover, Cul5 is important for the degradation of DAB1, a protein involved in the negative feedback mechanism during corticogenesis.

Researchers can use the Anti-Cul5/Cullin 5-Boster Bio antibody for research in breast cancer. These antibodies were developed to analyze HER2 expression in invasive BC and in normal breast tissue. The protein's expression was decreased in HER2pos breast cancer cell lines by lentiviral transduction. The cells expressing CUL5-transduced TUBO had upregulated MAPK and ErbB receptors.

The BC-box motif in the anti-Cul5/Cullin 5 antibodies from Boster Bio are responsible for facilitating E3 ubiquitin ligase activity in cells. The BC-box motif also plays an important role in facilitating the recruitment of E3 ubiquitin ligase into the fA3 cell membrane. This anti-Cul5 antibody is compatible with the other SOCS proteins.

The anti-Cul5/Cullin 5 (Boster Bio) is highly specific for Cul5 and neu. Neu-DC1 vaccination significantly retards tumor growth in BALB-HER2/neu transgenic mice. Tumor growth was monitored with MRI. The B-cells derived from neu-DC1-vaccinated mice secreted IFN-g. These results were assessed by an IFN-g ELISA.

The anti-Cul5/Cullin 5 antibodies from Boster Bio are highly effective for research. These antibodies have high affinity for Cul5 and Cullin and can be used to identify this protein in a range of cell cultures. The Boster Bio anti-Cul5/Cullin 5 antibody is suitable for use in Western blotting as well as in immunohistochemistry.

Data mining

There are three basic foundational stages in data mining. During these stages, the data is transformed into meaningful information, usually through the use of visualization tools. The CompTIA Whitepaper on Closing the Data Skills Gap outlines the three foundational stages in data activity. The information gained from these stages enables organizations to understand consumer behavior and develop products and services that better meet the needs of customers. It also helps them develop their marketing campaigns.

The data mining process seeks relationships between seemingly unrelated bits of information. Data is traditionally compartmentalized, making it difficult to analyze as a whole. For example, a company's performance may depend on the external factors affecting the performance of its products. For example, executives regularly look at sales numbers by product line, territory, distribution istspcina and seguonsh, bt the yof tn slck monstxteand can notdetecring wihythese sumbers boccured

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Anplications./h2>

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References

PMID: 9037604 by Byrd P.J., et al. Identification and analysis of expression of human VACM-1, a cullin gene family member located on chromosome 11q22-23.

PMID: 10230407 by Ohta T., et al. ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.