Anti-CTLA4 Monoclonal Antibody [1E6]

Product Name

Anti-CTLA4 Monoclonal Antibody [1E6]

View all CTLA-4 Antibodies

SKU/Catalog Number

M00020-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CTLA4 Monoclonal Antibody [1E6] (Catalog # M00020-1). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

CTLA-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CTLA4 Monoclonal Antibody [1E6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00020-1)

Host

Mouse

Contents

CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.

Clonality

Monoclonal

Clone Number

Clone: 1E6

Isotype

IgG1

Immunogen

CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of KLRA1 are known to exist; this antibody recognizes only the long isoform.

Reactive Species

M00020-1 is reactive to CTLA4 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

24656 MW

Background of CTLA-4

The cytotoxic T-lymphocyte-associated protein 4 (CTLA-4), also known as CD152, is a member of the immunoglobulin superfamily that is expressed by activated T cells and transmits an inhibitory signal to T cells (1,2). Both it and the homologous T-cell co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on antigen-presenting cells (APCs) (3). Mutations in the CTLA-4 gene have been implicated in multiple autoimmune diseases (4). CTLA-4 also functions as an immune checkpoint protein, and anti- CTLA-4 antibodies have been successfully used in the treatment of cancer (5).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00020-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 0.125 - 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CTLA4 (Source: Uniprot.org, NCBI)

Alternative Names

CTL4, cytotoxic T-lymphocyte associated protein 4, CD, GSE, GRD4, ALPS5, CD152, IDDM12, CELIAC3 CTLA4 ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12 cytotoxic T-lymphocyte associated protein 4 cytotoxic T-lymphocyte protein 4|celiac disease 3|cytotoxic T lymphocyte associated 4 short spliced form|cytotoxic T-lymphocyte-associated serine esterase-4|insulin-dependent diabetes mellitus 12|ligand and transmembrane spliced cytotoxic T lymphocyte associated 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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