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Product Info Summary
| SKU: | PB9493 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CTCF Antibody Picoband®
SKU/Catalog Number
PB9493
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CTCF Antibody Picoband® catalog # PB9493. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CTCF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9493)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CTCF recombinant protein (Position: K521-R727). Human CTCF shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat CTCF, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9493 is reactive to CTCF in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
140 kDa
Calculated molecular weight
82785 MW
Background of CTCF
Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. And this nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9493 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human 293T whole cell, human Caco-2 whole cell, rat C6 whole cell, mouse brain tissue, mouse RAW2647 whole cell
IHC: Mouse Intestine tissue, Rat Intestine tissue, Human Lung Cancer tissue
ICC/IF: U20S cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CTCF using anti-CTCF antibody (PB9493).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: rat C6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTCF antigen affinity purified polyclonal antibody (Catalog # PB9493) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTCF at approximately 140 kDa. The expected band size for CTCF is at 83 kDa.
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Figure 2. IHC analysis of CTCF using anti-CTCF antibody (PB9493).
CTCF was detected in a paraffin-embedded section of Mouse Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CTCF Antibody (PB9493) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of CTCF using anti-CTCF antibody (PB9493).
CTCF was detected in a paraffin-embedded section of Rat Intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CTCF Antibody (PB9493) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of CTCF using anti-CTCF antibody (PB9493).
CTCF was detected in a paraffin-embedded section of Human Lung Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CTCF Antibody (PB9493) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 5. IF analysis of CTCF using anti-CTCF antibody (PB9493).
CTCF was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CTCF Antibody (PB9493) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CTCF (Source: Uniprot.org, NCBI)
Gene Name
CTCF
Full Name
Transcriptional repressor CTCF
Weight
82785 MW
Superfamily
CTCF zinc-finger protein family
Alternative Names
Transcriptional repressor CTCF;11-zinc finger protein;CCCTC-binding factor;CTCFL paralog;CTCF; CTCF CFAP108, FAP108, MRD21 CCCTC-binding factor transcriptional repressor CTCF|11 zinc finger transcriptional repressor|11-zinc finger protein|CCCTC-binding factor (zinc finger protein)|CTCFL paralog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTCF, check out the CTCF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTCF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CTCF Antibody Picoband® (PB9493)
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No publications found for PB9493
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Customer Q&As
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5 Customer Q&As for Anti-CTCF Antibody Picoband®
Question
We have observed staining in mouse amniotic fluid. Any tips? Is anti-CTCF antibody supposed to stain amniotic fluid positively?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
From what I have seen in literature amniotic fluid does express CTCF. From what I have seen in Uniprot.org, CTCF is expressed in amniotic fluid, brain, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CTCF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15616553
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-26
Question
you antibody using your anti-CTCF antibody for negative regulation of cell population proliferation studies. Has this antibody been tested with western blotting on rat intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
We appreciate your inquiry. This PB9493 anti-CTCF antibody is validated on mouse intestine tissue, rat intestine tissue, lung cancer tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-21
Question
Our lab used your anti-CTCF antibody for WB on cervix carcinoma in a previous project. I am using human, and I plan to use the antibody for IHC next. My question regards examining cervix carcinoma as well as amniotic fluid in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
I took a look at the website and datasheets of our anti-CTCF antibody and it appears that PB9493 has been tested on human in both WB and IHC. Thus PB9493 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-03
Question
We were content with the WB result of your anti-CTCF antibody. However we have been able to see positive staining in brain nucleus using this antibody. Is that expected? Could you tell me where is CTCF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
From literature, brain does express CTCF. Generally CTCF expresses in nucleus, nucleoplasm. Regarding which tissues have CTCF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15616553
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-05-27
Question
We are currently using anti-CTCF antibody PB9493 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
The anti-CTCF antibody (PB9493) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-14
