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Product Info Summary
| SKU: | A03240 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-PEG10 Monoclonal Antibody
SKU/Catalog Number
A03240
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PEG10 Monoclonal Antibody catalog # A03240. Tested in IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PEG10 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03240)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1B1C4
Isotype
IgG
Immunogen
Purified recombinant fragment of PEG10 (aa1-120) expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03240 is reactive to PEG10 in Human
Applications
A03240 is guaranteed for IF, IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
80173 MW
Background of PEG10
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded human Placenta tissues with AEC staining using PEG10 Monoclonal antibody.
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Western Blot (WB) analysis using PEG10 Monoclonal antibody against truncated Trx-PEG10 recombinant protein (1),truncated GST-PEG10 (aa1-120) recombinant protein (2) and full-length PEG10 (aa1-325)-hIgGFc transfected CHO-K1 cell lysate (3).
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Confocal Immunofluorescence (IF) analysis of methanol-fixed HepG2 cells using PEG10 Monoclonal antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Protein Target Info & Infographic
Gene/Protein Information For PEG10 (Source: Uniprot.org, NCBI)
Gene Name
PEG10
Full Name
Retrotransposon-derived protein PEG10
Weight
80173 MW
Alternative Names
EDR; embryonal carcinoma differentiation regulated; Embryonal carcinoma differentiation-regulated protein; HB-1; KIAA1051EDR; Mammalian retrotransposon-derived protein 2; Mar2; Mart2; MEF3 Like 1; MEF3L; MEF3L1; Myelin expression factor 3-like protein 1; paternally expressed 10; Paternally expressed gene 10 protein; PEG10; retrotransposon gag domain containing 3; Retrotransposon gag domain-containing protein 3; Retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; RGAG3; RGAG3MEF3-like protein 1; RTL2; SIRH1; Ty3/Gypsy-like protein PEG10 EDR, HB-1, MEF3L, Mar2, Mart2, RGAG3, RTL2, SIRH1 paternally expressed 10 retrotransposon-derived protein PEG10|MEF3 like 1|Sushi-Ichi retrotransposon homolog 1|embryonal carcinoma differentiation regulated|mammalian retrotransposon-derived 2|mammalian retrotransposon-derived protein 2|myelin expression factor 3-like protein 1|paternally expressed gene 10 protein|retrotransposon Gag like 2|retrotransposon gag domain-containing protein 3|retrotransposon-derived gag-like polyprotein|ty3/Gypsy-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PEG10, check out the PEG10 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PEG10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PEG10 Monoclonal Antibody (A03240)
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