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Product Info Summary
| SKU: | A00132 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Transcriptional repressor CTCF CTCF Antibody
SKU/Catalog Number
A00132
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Transcriptional repressor CTCF CTCF Antibody catalog # A00132. Tested in WB,ICC/IF,IHC,IP applications. This antibody reacts with Human,Mouse,Rat,Zebrafish.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Transcriptional repressor CTCF CTCF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00132)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00132 is reactive to CTCF in Human, Mouse, Rat, Zebrafish
Reconstitution
Calculated molecular weight
82785 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00132 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC: 1:50-1:200
IP: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of CTCF using Anti-Transcriptional repressor CTCF CTCF Antibody (A00132).
ICC staining CTCF in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Transcriptional repressor CTCF CTCF Antibody A00132
Western blot analysis of CTCF on different cell lysates using anti-CTCF antibody at 1/500 dilution. Positive control: Lane 1: 293T Lane 2:MCF-7Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CTCF (Source: Uniprot.org, NCBI)
Gene Name
CTCF
Full Name
Transcriptional repressor CTCF
Weight
82785 MW
Superfamily
CTCF zinc-finger protein family
Alternative Names
Transcriptional repressor CTCF;11-zinc finger protein;CCCTC-binding factor;CTCFL paralog;CTCF; CTCF CFAP108, FAP108, MRD21 CCCTC-binding factor transcriptional repressor CTCF|11 zinc finger transcriptional repressor|11-zinc finger protein|CCCTC-binding factor (zinc finger protein)|CTCFL paralog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTCF, check out the CTCF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTCF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcriptional repressor CTCF CTCF Antibody (A00132)
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No publications found for A00132
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