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Product Info Summary
| SKU: | A03301 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-COLQ Antibody
SKU/Catalog Number
A03301
Size
100ul
Form
Liquid
Description
Boster Bio Anti-COLQ Antibody (Catalog# A03301). Tested in IHC-P, IF (paraffin), WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-COLQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03301)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human COLQ. Immunogen sequence location: 189 - 238
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A03301 is reactive to COLQ in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
72 kDa
Calculated molecular weight
47766 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03301 is guaranteed for IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50 - 1:200
Western Blot, 1:500 - 1:2000
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells , using COLQ antibody.
Protein Target Info & Infographic
Gene/Protein Information For COLQ (Source: Uniprot.org, NCBI)
Gene Name
COLQ
Full Name
Acetylcholinesterase collagenic tail peptide
Weight
47766 MW
Superfamily
COLQ family
Alternative Names
Acetylcholinesterase collagenic tail peptide (AChE Q subunit) (Acetylcholinesterase-associated collagen) COLQ CMS5, EAD collagen like tail subunit of asymmetric acetylcholinesterase acetylcholinesterase collagenic tail peptide|AChE Q subunit|acetylcholinesterase-associated collagen|collagen-like tail subunit (single strand of homotrimer) of asymmetric acetylcholinesterase|collagenic tail of endplate acetylcholinesterase|single strand of homotrimeric collagen-like tail subunit of asymmetric acetylcholinesterase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COLQ, check out the COLQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COLQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-COLQ Antibody (A03301)
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No publications found for A03301
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-COLQ Antibody
Question
I have a question about product A03301, anti-COLQ antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03301 anti-COLQ antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
Would A03301 anti-COLQ antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Anderson
Verified customer
Asked: 2019-04-12
Answer
As indicated on the product datasheet, A03301 anti-COLQ antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-04-12
Question
We are currently using anti-COLQ antibody A03301 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
The anti-COLQ antibody (A03301) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-07
Question
Is a blocking peptide available for product anti-COLQ antibody (A03301)?
K. Krishna
Verified customer
Asked: 2015-02-13
Answer
We do provide the blocking peptide for product anti-COLQ antibody (A03301). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-02-13
Question
I was wanting to use your anti-COLQ antibody for WB for rat skeletal muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat skeletal muscle identification?
Z. Thomas
Verified customer
Asked: 2013-04-29
Answer
You can see on the product datasheet, A03301 anti-COLQ antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skeletal muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-29
