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Product Info Summary
| SKU: | A01170-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Collagen VII/COL7A1 Antibody Picoband®
View all Collagen VII Antibodies
SKU/Catalog Number
A01170-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen VII/COL7A1 Antibody Picoband® catalog # A01170-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen VII/COL7A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01170-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Collagen VII/COL7A1 recombinant protein (Position: E2764-D2944).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01170-1 is reactive to COL7A1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
300 kDa
Calculated molecular weight
295.22kDa
Background of Collagen VII
Collagen alpha-1 (VII) chain is a protein that in humans is encoded by the COL7A1 gene. This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01170-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human Hela whole cell
IHC: human melanoma tissue, human tonsil tissue
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Collagen VII/COL7A1 using anti-Collagen VII/COL7A1 antibody (A01170-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen VII/COL7A1 antigen affinity purified polyclonal antibody (Catalog # A01170-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen VII/COL7A1 at approximately 300KD. The expected band size for Collagen VII/COL7A1 is at 300KD.
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Figure 2. Flow Cytometry analysis of A549 cells using anti-Collagen VII/COL7A1 antibody (A01170-1).
Overlay histogram showing A549 cells stained with A01170-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Collagen VII/COL7A1 Antibody (A01170-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. IHC analysis of Collagen VII/COL7A1 using anti-Collagen VII/COL7A1 antibody (A01170-1).
Collagen VII/COL7A1 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Collagen VII/COL7A1 Antibody (A01170-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of Collagen VII/COL7A1 using anti-Collagen VII/COL7A1 antibody (A01170-1).
Collagen VII/COL7A1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Collagen VII/COL7A1 Antibody (A01170-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For COL7A1 (Source: Uniprot.org, NCBI)
Gene Name
COL7A1
Full Name
Collagen alpha-1(VII) chain
Weight
295.22kDa
Alternative Names
Placenta growth factor; PlGF; PGFL; PLGF; PGF COL7A1 EBD1, EBDCT, EBR1, NDNC8 collagen type VII alpha 1 chain collagen alpha-1(VII) chain|LC collagen|collagen VII, alpha-1 polypeptide|collagen, type VII, alpha 1|long-chain collagen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL7A1, check out the COL7A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL7A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen VII/COL7A1 Antibody Picoband® (A01170-1)
Hello CJ!
A01170-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CDDO-Im ameliorates osteoarthritis and inhibits chondrocyte apoptosis in mice via enhancing Nrf2-dependent autophagy
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties
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Customer Q&As
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1 Customer Q&As for Anti-Collagen VII/COL7A1 Antibody Picoband®
Question
What antibdy is recommended for Western Blot for Collagen type VII?
Verified customer
Asked: 2021-02-23
Answer
We recommend the Anti-Collagen VII/COL7A1 Antibody Picoband™ (A01170-1) which was validated using Hela cell.
Boster Scientific Support
Answered: 2021-02-23
