Click image to see more details
Product Info Summary
| SKU: | A00517 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Bovine, Human |
| Host: | Rabbit |
| Application: | Dot blot, IHC |
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Product info
Product Name
Anti-Collagen Type II COL2A1 Antibody
View all Collagen II Antibodies
SKU/Catalog Number
A00517
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Collagen Type II COL2A1 Antibody (Catalog # A00517). Tested in Dot blot, IHC applications. This antibody reacts with Human, Bovine.
Storage & Handling
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Collagen Type II COL2A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00517)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Collagen Type II from human knee cartilage and bovine nasal cartilage.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00517 is reactive to COL2A1 in Bovine, Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
141785 MW
Background of Collagen II
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of ‘type’ specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Boster extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00517 is guaranteed for Dot blot, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:5,000 - 1:50,000
IHC: 1:50 - 1:400
IF Microscopy: User optimized
IP: 1:100
WB: 1:1,000 - 1:10,000
Validation Images & Assay Conditions
Click image to see more details
Collagen II was detected in paraffin-embedded sections of human bronchiolar cartilage tissue using rabbit anti-Collagen Type II Antigen Affinity purified polyclonal antibody (Catalog # A00517) at 1:400. Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
Protein Target Info & Infographic
Gene/Protein Information For COL2A1 (Source: Uniprot.org, NCBI)
Gene Name
COL2A1
Full Name
Collagen alpha-1(II) chain
Weight
141785 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen Type II, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen, Chondrocalcin, COL2A1 COL2A1 ANFH, AOM, COL11A3, SEDC, STL1 collagen type II alpha 1 chain collagen alpha-1(II) chain|alpha-1 type II collagen|arthroophthalmopathy, progressive (Stickler syndrome)|cartilage collagen|chondrocalcin|collagen II, alpha-1 polypeptide|collagen, type II, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL2A1, check out the COL2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen Type II COL2A1 Antibody (A00517)
Hello CJ!
A00517 has been cited in 26 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Resveratrol inhibits the IL-1β-induced expression of MMP-13 and IL-6 in human articular chondrocytes via TLR4/MyD88-dependent and -independent signaling cascades
Salah MM,Ashour AA,Abdelghany TM,Abdel-Aziz AH,Salama SA.Pirfenidone alleviates concanavalin A-induced liver fibrosis in mice.Life Sci.2019 Dec 15;239:116982.doi:10.1016/j.lfs.2019.116982.Epub 2019 Oct 19.PMID:31639402.
Species: Mouse
A00517 usage in article: APP:IHC, SAMPLE:LIVER TISSUE, DILUTION:NA
Guo Z,Qiu C,Mecca C,Zhang Y,Bian J,Wang Y,Wu X,Wang T,Su W,Li X,Zhang W,Chen B,Xiang H.Elevated lymphotoxin-α (TNFβ) is associated with intervertebral disc degeneration.BMC Musculoskelet Disord.2021 Jan 13;22(1):77.doi:10.1186/s12891-020-03934-7.PMID:3344
Species: Human,Rat
A00517 usage in article: APP:IF, SAMPLE:NP CELL, DILUTION:1:100
Zhang Q,Xiang W,Xue BZ,Yi DY,Zhao HY,Fu P. Growth factors contribute to the mediation of angiogenic capacity of glioma-associated mesenchymal stem cells. Oncol Lett. 2021 Mar;21(3):215.doi: 10.3892/ol.2021.12476. Epub 2021 Jan 19.PMID:33552293;PMCID:PMC78
Species: Human
Chaoyue Hong,Yi Tang,Xiu Hu,Xiwen Song,Yun Cai,Zhaohui Song,Feiwu Kang,Partial deficiency of HIF-1α in chondrocytes effected bone repair of mandibular condylar neck, Archives of Oral Biology, 2020, 105023, ISSN 0003-9969, https://doi.org/10.1016/j.archora
Species: Mouse
A00517 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:100
Ma K,Zhu B,Wang Z,Cai P,He M,Ye D,Yan G,Zheng L,Yang L,Zhao J.Articular chondrocyte-derived extracellular vesicles promote cartilage differentiation of human umbilical cord mesenchymal stem cells by activation of autophagy.J Nanobiotechnology.2020 Nov 9;1
Species: Human,New Zealand Rabbit
A00517 usage in article: APP:IF, SAMPLE:HUCMSCS, DILUTION:1:200
Zhang P,Chang BG.Inhibitory effect on the apoptosis of disc nucleus pulposus cells in rats by silencing COL9A3 gene to mediate MAPK signaling pathway: a study on the function and mechanism.Eur Rev Med Pharmacol Sci.2020 Sep;24(17):8653-8664.doi:10.26355/e
Species: Rat
A00517 usage in article: APP:IHC, SAMPLE:NUCLEUS PULPOSUS CELL, DILUTION:1:200
Lan Q,Lu R,Chen H,Pang Y, Xiong F,Shen C,Qin Z,Zheng L,Xu G,Zhao J.MMP-13 enzyme and pH responsive theranostic nanoplatform for osteoarthritis.J Nanobiotechnology.2020 Aug 27;18(1):117.doi:10.1186/s12951-020-00666-7. PMID:32854712;PMCID:PMC7450974.
Species: Mouse
A00517 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:200
Sun M,Lu Z,Cai P,Zheng L,Zhao J.Salidroside enhances proliferation and maintains phenotype of articular chondrocytes for autologous chondrocyte implantation (ACI) via TGF-β/Smad3 Signal.Biomed Pharmacother.2020 Feb;122:109388.doi:10.1016/j.biopha.2019.109
Species: Rat
A00517 usage in article: APP:IF, SAMPLE:CHONDROCYTES, DILUTION:1:200
Ni B,Huang X,Xi Y,Mao Z,Chu X,Zhang R,Ma X,You H.Neferine Inhibits Expression of Inflammatory Mediators and Matrix Degrading Enzymes in IL-1β-Treated Rat Chondrocytes via Suppressing MAPK and NF-κB Signaling Pathways.Inflammation.2020 Aug;43(4):1209-1221.
Species: Rat
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Collagen Type II COL2A1 Antibody
Question
Would A00517 anti-Collagen Type II antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, A00517 anti-Collagen Type II antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-27
Question
We were satisfied with the WB result of your anti-Collagen Type II antibody. However we have been able to see positive staining in fetal sternum secreted using this antibody. Is that expected? Could you tell me where is COL2A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
From what I have seen in literature, fetal sternum does express COL2A1. Generally COL2A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2020-01-17
Question
We have seen staining in human blood. Are there any suggestions? Is anti-Collagen Type II antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
Based on literature blood does express COL2A1. Based on Uniprot.org, COL2A1 is expressed in tibia, blood, embryonic stem cell muscle, fetal sternum, among other tissues. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-Collagen Type II antibody for IHC for mouse embryonic stem cell muscle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic stem cell muscle identification?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
You can see on the product datasheet, A00517 anti-Collagen Type II antibody has been validated for IP, IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic stem cell muscle in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-12
Question
Will anti-Collagen Type II antibody A00517 work for IHC with embryonic stem cell muscle?
H. Jackson
Verified customer
Asked: 2019-10-17
Answer
According to the expression profile of embryonic stem cell muscle, COL2A1 is highly expressed in embryonic stem cell muscle. So, it is likely that anti-Collagen Type II antibody A00517 will work for IHC with embryonic stem cell muscle.
Boster Scientific Support
Answered: 2019-10-17
Question
Is a blocking peptide available for product anti-Collagen Type II antibody (A00517)?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
We do provide the blocking peptide for product anti-Collagen Type II antibody (A00517). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-24
Question
See below the WB image, lot number and protocol we used for embryonic stem cell muscle using anti-Collagen Type II antibody A00517. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-16
Question
Is this A00517 anti-Collagen Type II antibody reactive to the isotypes of COL2A1?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The immunogen of A00517 anti-Collagen Type II antibody is Collagen Type II from human knee cartilage and bovine nasal cartilage. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-27
Question
I see that the anti-Collagen Type II antibody A00517 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-16
Question
I was wanting to use to test anti-Collagen Type II antibody A00517 on mouse embryonic stem cell muscle for research purposes, then I may be interested in using anti-Collagen Type II antibody A00517 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
The products we sell, including anti-Collagen Type II antibody A00517, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-20
Question
Our lab used your anti-Collagen Type II antibody for IHC on embryonic stem cell muscle last year. I am using human, and We intend to use the antibody for IP next. We are interested in examining embryonic stem cell muscle as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-08-14
Answer
I looked at the website and datasheets of our anti-Collagen Type II antibody and it seems that A00517 has been tested on human in both IHC and IP. Thus A00517 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-08-14
Question
We are currently using anti-Collagen Type II antibody A00517 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-19
Answer
The anti-Collagen Type II antibody (A00517) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-19
Question
Do you have a BSA free version of anti-Collagen Type II antibody A00517 available?
B. Walker
Verified customer
Asked: 2018-06-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Collagen Type II antibody A00517 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-26
Question
I am interested in using your anti-Collagen Type II antibody for tissue homeostasis studies. Has this antibody been tested with western blotting on bronchiolar cartilage tissue? We would like to see some validation images before ordering.
T. Lewis
Verified customer
Asked: 2015-05-27
Answer
I appreciate your inquiry. This A00517 anti-Collagen Type II antibody is tested on bronchiolar cartilage tissue. It is guaranteed to work for IP, IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-05-27
Question
I have a question about product A00517, anti-Collagen Type II antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Lewis
Verified customer
Asked: 2014-06-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00517 anti-Collagen Type II antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-06-18
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic stem cell muscle using anti-Collagen Type II antibody A00517. Let me know if you need anything else.
N. Parker
Verified customer
Asked: 2013-06-12
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-12
