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Product Info Summary
| SKU: | PA2141 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Collagen II/COL2A1 Antibody Picoband®
View all Collagen II Antibodies
SKU/Catalog Number
PA2141
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Collagen II/COL2A1 Antibody catalog # PA2141. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen II/COL2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2141)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II, different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2141 is reactive to COL2A1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170 kDa
Calculated molecular weight
141785 MW
Background of Collagen II
Collagen, type II, alpha 1, also known as COL2A1, is a human gene which is the major collagen synthesized by chondrocvtes. COL2A1 is mapped to 12q13.11. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2141 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: COLO320 Whole Cell, HELA Whole Cell, MCF-7Whole Cell, A549 Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
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Anti-COL2A1 antibody, PA2141, Western blotting
All lanes: Anti COL2A1 (PA2141) at 0.5ug/ml
Lane 1: COLO320 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: MCF-7Whole Cell Lysate at 40ug
Lane 4: A549 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 134 KD
Observed bind size: 170KD
Protein Target Info & Infographic
Gene/Protein Information For COL2A1 (Source: Uniprot.org, NCBI)
Gene Name
COL2A1
Full Name
Collagen alpha-1(II) chain
Weight
141785 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-1 (II) chain;Alpha-1 type II collagen;Collagen alpha-1 (II) chain;Chondrocalcin;COL2A1; COL2A1 ANFH, AOM, COL11A3, SEDC, STL1 collagen type II alpha 1 chain collagen alpha-1(II) chain|alpha-1 type II collagen|arthroophthalmopathy, progressive (Stickler syndrome)|cartilage collagen|chondrocalcin|collagen II, alpha-1 polypeptide|collagen, type II, alpha 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL2A1, check out the COL2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen II/COL2A1 Antibody Picoband® (PA2141)
Hello CJ!
PA2141 has been cited in 31 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of Angelicae Pubescentis and Loranthi Decotion on repairing knee joint cartilages in rats
Microfluidics-based optimization of neuroleukin-mediated regulation of articular chondrocyte proliferation
Nano-hydroxyapatite/collagen film as a favorable substrate to maintain the phenotype and promote the growth of chondrocytes cultured in vitro
(+)-Cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells by activating vitamin D receptor
Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/?-catenin pathway activity
Establishment of a rat model for osteoarthritis resulting from anterior cruciate ligament rupture and its significance
Neuroleukin/Autocrine Motility Factor Receptor Pathway Promotes Proliferation of Articular Chondrocytes through Activation of AKT and Smad2/3
Construction of tissue-engineered laryngeal cartilage with a hollow, semi-flared shape using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) as a scaffold.
Hypoxia Enhances Protective Effect of Placental-Derived Mesenchymal Stem Cells on Damaged Intestinal Epithelial Cells by Promoting Secretion of Insulin-Like Growth Factor-1
Protective effects of baicalin on rabbit articular chondrocytes in vitro
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Collagen II/COL2A1 Antibody Picoband®
Question
Would anti-Collagen II/COL2A1 antibody PA2141 work on dog WB with tibia?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
Our lab technicians have not tested anti-Collagen II/COL2A1 antibody PA2141 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Collagen II/COL2A1 antibody PA2141 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog tibia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal sternum using anti-Collagen II/COL2A1 antibody PA2141. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-12
Question
Here is the WB image, lot number and protocol we used for fetal sternum using anti-Collagen II/COL2A1 antibody PA2141. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-21
Question
My question regards to test anti-Collagen II/COL2A1 antibody PA2141 on rat fetal sternum for research purposes, then I may be interested in using anti-Collagen II/COL2A1 antibody PA2141 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The products we sell, including anti-Collagen II/COL2A1 antibody PA2141, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-28
Question
We have observed staining in rat fetal sternum. Are there any suggestions? Is anti-Collagen II/COL2A1 antibody supposed to stain fetal sternum positively?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
From what I have seen in literature fetal sternum does express COL2A1. From what I have seen in Uniprot.org, COL2A1 is expressed in tibia, blood, embryonic stem cell muscle, fetal sternum, among other tissues. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2019-11-01
Question
My team were content with the WB result of your anti-Collagen II/COL2A1 antibody. However we have been able to see positive staining in blood extracellular using this antibody. Is that expected? Could you tell me where is COL2A1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
From what I have seen in literature, blood does express COL2A1. Generally COL2A1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have COL2A1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 8948452
Embryonic stem cell, and Muscle, Pubmed ID: 15489334
Fetal sternum, Pubmed ID: 1999183
Boster Scientific Support
Answered: 2019-07-19
Question
I was wanting to use your anti-Collagen II/COL2A1 antibody for WB for rat fetal sternum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat fetal sternum identification?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
It shows on the product datasheet, PA2141 anti-Collagen II/COL2A1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal sternum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-03
Question
Is there a BSA free version of anti-Collagen II/COL2A1 antibody PA2141 available?
K. Johnson
Verified customer
Asked: 2019-06-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Collagen II/COL2A1 antibody PA2141 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-20
Question
Our lab want to know about using your anti-Collagen II/COL2A1 antibody for collagen chain trimerization studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-04-10
Answer
We appreciate your inquiry. This PA2141 anti-Collagen II/COL2A1 antibody is validated on colo320 whole cell lysate, hela whole cell lysate, a549 whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-04-10
Question
I see that the anti-Collagen II/COL2A1 antibody PA2141 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-04-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-04-04
Question
My question regarding product PA2141, anti-Collagen II/COL2A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2141 anti-Collagen II/COL2A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-23
Question
Is this PA2141 anti-Collagen II/COL2A1 antibody reactive to the isotypes of COL2A1?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
The immunogen of PA2141 anti-Collagen II/COL2A1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen II(1222-1241aa AFAGLGPREKGPDPLQYMRA), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-14
Question
Does anti-Collagen II/COL2A1 antibody PA2141 work for WB with fetal sternum?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
According to the expression profile of fetal sternum, COL2A1 is highly expressed in fetal sternum. So, it is likely that anti-Collagen II/COL2A1 antibody PA2141 will work for WB with fetal sternum.
Boster Scientific Support
Answered: 2017-06-01
Question
Is a blocking peptide available for product anti-Collagen II/COL2A1 antibody (PA2141)?
J. Collins
Verified customer
Asked: 2016-12-06
Answer
We do provide the blocking peptide for product anti-Collagen II/COL2A1 antibody (PA2141). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-12-06
Question
We are currently using anti-Collagen II/COL2A1 antibody PA2141 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Z. Baker
Verified customer
Asked: 2015-11-05
Answer
The anti-Collagen II/COL2A1 antibody (PA2141) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-05
Question
Does PA2141 anti-Collagen II/COL2A1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Evans
Verified customer
Asked: 2013-05-13
Answer
As indicated on the product datasheet, PA2141 anti-Collagen II/COL2A1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-05-13
