Product Info Summary
SKU: | M00329-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC-P, ICC |
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Product info
Product Name
Anti-Collagen I COL1A1 Monoclonal Antibody
SKU/Catalog Number
M00329-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Collagen I COL1A1 Monoclonal Antibody catalog # M00329-1. Tested in IF, IHC-P, ICC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen I COL1A1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00329-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
1F12
Isotype
IgG
Immunogen
Synthetic Peptide of Collagen I
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00329-1 is reactive to COL1A1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
138941 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00329-1 is guaranteed for IF, IHC-P, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P 1:50-300
IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human skin.
Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue.
Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human skin.
Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human skin.
Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat kidney tissue.
Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Protein Target Info & Infographic
Gene/Protein Information For COL1A1 (Source: Uniprot.org, NCBI)
Gene Name
COL1A1
Full Name
Collagen alpha-1(I) chain
Weight
138941 MW
Superfamily
fibrillar collagen family
Alternative Names
Collagen alpha-1 (I) chain;Alpha-1 type I collagen;COL1A1; COL1A1 CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4 collagen type I alpha 1 chain collagen alpha-1(I) chain|alpha-1 type I collagen|alpha1(I) procollagen|collagen alpha 1 chain type I|collagen alpha-1(I) chain preproprotein|collagen of skin, tendon and bone, alpha-1 chain|collagen, type I, alpha 1|pro-alpha-1 collagen type 1|type I proalpha 1|type I procollagen alpha 1 chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL1A1, check out the COL1A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL1A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen I COL1A1 Monoclonal Antibody (M00329-1)
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