Product Info Summary
SKU: | M00220 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, Functional Assay |
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Product info
Product Name
Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody
SKU/Catalog Number
M00220
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody (Catalog # M00220). Tested in Functional Assay, Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00220)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: BU63
Isotype
IgG1, kappa
Immunogen
ARH-77 (B-lymphoblastoid cell line)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00220 is reactive to CD86 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
37682 MW
Background of B7-2/CD86
Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00220 is guaranteed for Flow Cytometry, IF, IHC, Functional Assay Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional Studies (Order Ab without Azide)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Flow Cytometric Analysis of human PBMCs using Anti-CD86 Mouse Monoclonal Antibody (BU63); Goat anti-Mouse IgG-CF488 (red); Isotype Control (green).
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Formalin-fixed, paraffin-embedded human Esophageal Tumor stained with Anti-CD86 Mouse Monoclonal Antibody (BU63).
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Immunofluorescence staining of PFA-fixed Ramos cells using Anti-CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
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Flow Cytometric analysis of PFA-fixed Ramos cells. Anti-CD86 Mouse Monoclonal Antibody (BU63) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Target Info & Infographic
Gene/Protein Information For CD86 (Source: Uniprot.org, NCBI)
Gene Name
CD86
Full Name
T-lymphocyte activation antigen CD86
Weight
37682 MW
Alternative Names
T-lymphocyte activation antigen CD86;Activation B7-2 antigen;B70;BU63;CTLA-4 counter-receptor B7.2;FUN-1;CD86;CD86;CD28LG2; CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation CD86|B-lymphocyte activation B7-2|BU63|CD86 (CD28 ligand 2, B7-2 )|CTLA-4 counter-receptor B7.2|FUN-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD86, check out the CD86 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD86: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody (M00220)
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Customer Q&As
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4 Customer Q&As for Anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal Antibody
Question
My boss were happy with the WB result of your anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD86 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Based on literature, brain does express CD86. Generally CD86 expresses in cell membrane. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777
Boster Scientific Support
Answered: 2020-04-08
Question
Our lab used your anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal antibody for IP on leukocyte a few months ago. I am using mouse, and I plan to use the antibody for Flow Cytometry next. Our lab want to know about examining leukocyte as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
I looked at the website and datasheets of our anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal antibody and it seems that M00220 has been tested on mouse in both IP and Flow Cytometry. Thus M00220 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-17
Question
We have seen staining in mouse leukocyte. Any tips? Is anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2017-11-08
Answer
From what I have seen in literature leukocyte does express CD86. From what I have seen in Uniprot.org, CD86 is expressed in leukocyte, brain, foreskin, among other tissues. Regarding which tissues have CD86 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039, 15489334
Foreskin, Pubmed ID: 7541777
Boster Scientific Support
Answered: 2017-11-08
Question
We are currently using anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal antibody M00220 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
V. Li
Verified customer
Asked: 2013-12-10
Answer
The anti-CD86 (Dendritic Cells Maturation Marker) Monoclonal antibody (M00220) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-12-10