Anti-CLASP2 Antibody Picoband™

Product Name

Anti-CLASP2 Antibody Picoband™

View all CLASP2 Antibodies

SKU/Catalog Number

A03579-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CLASP2 Antibody Picoband™ catalog # A03579-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CLASP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03579-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CLASP2 recombinant protein (Position: A266-D1230).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03579-1 is reactive to CLASP2 in Human, Mouse, Rat

Applications

A03579-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

165 kDa

Calculated molecular weight

78055 MW

Background of CLASP2

CLASP2 (CLIP-associating protein 2) is a brain specific protein belongs to CLASP family. CLASP1 and CLASP2 regulate microtubule plus-end dynamics in brine cortex. CLASP2 is required for the orientation of stabilized microtubules toward the leading edge, It may act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms. Phosphorylation of CLASP2 by GSK3B may negatively regulate binding to microtubule lattices in lamella.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CLASP2 using anti-CLASP2 antibody (A03579-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEK293 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: human HL-60 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLASP2 antigen affinity purified polyclonal antibody (Catalog # A03579-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLASP2 at approximately 165 kDa. The expected band size for CLASP2 is at 141 kDa.

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Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CLASP2 antibody (A03579-1).
Overlay histogram showing THP-1 cells stained with A03579-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CLASP2 Antibody (A03579-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Figure 3. IF analysis of CLASP2 using anti-CLASP2 antibody (A03579-1).
CLASP2 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CLASP2 Antibody (A03579-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Gene/Protein Information For CLASP2 (Source: Uniprot.org, NCBI)

Alternative Names

CLIP-associating protein 2; cytoplasmic linker associated protein 2; Cytoplasmic linker-associated protein 2; hOrbit2; KIAA0627; multiple asters (Mast)-like homolog 2; Protein Orbit homolog 2 CLASP2 cytoplasmic linker associated protein 2 CLIP-associating protein 2|CLIP-associating protein CLASP2|multiple asters (Mast)-like homolog 2|protein Orbit homolog 2

For more info on CLASP2, check out the CLASP2 Infographic

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In this infographic, you will see the following information for CLASP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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