Click image to see more details
Product Info Summary
| SKU: | A02404-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Dishevelled 2/DVL2 Antibody Picoband®
View all Dishevelled-2 Antibodies
SKU/Catalog Number
A02404-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dishevelled 2/DVL2 Antibody Picoband® catalog # A02404-3. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dishevelled 2/DVL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02404-3)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Dishevelled 2, identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02404-3 is reactive to DVL2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
79 kDa
Calculated molecular weight
78948 MW
Background of Dishevelled-2
Segment polarity protein dishevelled homolog DVL-2 is a protein that in humans is encoded by the DVL2 gene. This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02404-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: rat liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Dishevelled 2 using anti-Dishevelled 2 antibody (A02404-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dishevelled 2 antigen affinity purified polyclonal antibody (Catalog # A02404-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Dishevelled 2 at approximately 79KD. The expected band size for Dishevelled 2 is at 79KD.
Protein Target Info & Infographic
Gene/Protein Information For DVL2 (Source: Uniprot.org, NCBI)
Gene Name
DVL2
Full Name
Segment polarity protein dishevelled homolog DVL-2
Weight
78948 MW
Superfamily
DSH family
Alternative Names
Segment polarity protein dishevelled homolog DVL-2;Dishevelled-2;DSH homolog 2;DVL2; DVL2 dishevelled segment polarity protein 2 segment polarity protein dishevelled homolog DVL-2|dishevelled 2 (homologous to Drosophila dsh)|dishevelled, dsh homolog 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DVL2, check out the DVL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DVL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Dishevelled 2/DVL2 Antibody Picoband® (A02404-3)
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Customer Q&As
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6 Customer Q&As for Anti-Dishevelled 2/DVL2 Antibody Picoband®
Question
My question regarding product A02404-3, anti-Dishevelled 2/DVL2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02404-3 anti-Dishevelled 2/DVL2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-16
Question
I was wanting to use your anti-Dishevelled 2/DVL2 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
It shows on the product datasheet, A02404-3 anti-Dishevelled 2/DVL2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-04
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Dishevelled 2/DVL2 antibody A02404-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-01
Question
Will anti-Dishevelled 2/DVL2 antibody A02404-3 work on pig WB with vagina?
R. Anderson
Verified customer
Asked: 2019-09-24
Answer
Our lab technicians have not validated anti-Dishevelled 2/DVL2 antibody A02404-3 on pig. You can run a BLAST between pig and the immunogen sequence of anti-Dishevelled 2/DVL2 antibody A02404-3 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig vagina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-24
Question
We are currently using anti-Dishevelled 2/DVL2 antibody A02404-3 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
The anti-Dishevelled 2/DVL2 antibody (A02404-3) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-27
Question
I see that the anti-Dishevelled 2/DVL2 antibody A02404-3 works with WB, what is the protocol used to produce the result images on the product page?
R. Patel
Verified customer
Asked: 2016-05-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-05-13
